rDNA sequence 비교를 통한 한국유래 Kudoa septempunctata와 일본 유래 Kudoa septempunctata의 구분 maker 개발 가능성에 대한 연구

Abstract

본 연구에서는 한국 유래 K. septempunctata와 일본 유래 K. septempunctata의 유전적 차이를 확인하기 위하여 ITS seqeunce를 비교하였고, 비교 결과 한국 유래와 일본 유래 K. septempunctata의 차이를 확인할 수 있었다. 이러한 결과는 향후 한국 유래 K. septempunctata와 일본 유래 K. septempunctata를 구별할 수 있는 방법 개발에 기초적인 연구가 될 것으로 추정한다.

Kudoa septempunctata, is a MYXOSPOREAN parasite that infect trunk muscles of olive flounder (Paralichthys olivaceus). To dates, Olive flounder aquafarm industry has been suffering a huge economic loss caused by this parasitic infection. Reason findings demonstrated that this parasite can infect to humans and cause food poisoning. Therefore detection of K. septempunctata at the initial stage of the infection is useful to develop proper disease management strategies. K. septempunctata has three distinct genotypes, ST1, ST2 and ST3, which could be distinguished by presence of ribosomal internal transcribed spacer (ITS) regions situated between small-subunit rRNA and large subunit rRNA. Typically, ST1 and ST2 genotypes can be identified in Japan, while ST3 known to be found in Korea. This study was focused to develop the PCR based method to distinguish K. septempunctata local difference using ITS sequences. PCR primers were designed based on rDNA sequences of K. septempunctata followed by PCR was preformed using genomic DNA extracted from K. septempunctata infected olive flounders obtained from Korea and Japan. According to the cloning results, rDNA sequence difference of the Korean and Japanese originated K. septempunctata were confirmed. Therefore we suggest, this method is reliable for distinguish the K. septempunctata local difference.