Growth and lipid accumulation by oceanic microalga Chlorella sp. CKC2 in mammalian cell culture waste supplemented medium

Abstract

Currently, microalgae considered as one of the potent resources for the sustainable biodiesel production. To enhance biodiesel yield by indigenous microalgae via cost-effective process, this study was investigated availability of mammalian cell culture waste (MCCW) as an additive to culture medium. To evaluate growth response of microalgae, aseptically isolated Chlorella sp. CKC2 was cultivated in different concentrations (v/v, 0-100%) of MCCW mixed with F/2 medium. As results, maximum 6.2-fold higher dry cell weight (DCW) of Chlorella sp. was obtained at 20% compared to control (0%), and concentrations-dependently decreased DCW was observed upper concentrations. In the result of further growth test under 0, 5, 10, and 20%, MCCW concentration-dependently increased daily growth patterns were observed along with gradually increased DCW. Furthermore, more significantly augmented lipid productivities were obtained from MCCW supplemented culture with high accumulation of polyunsaturated fatty acid methyl esters compared to control (0%) which indicated changes of biodiesel properties. From the results of this study, it is suggested that MCCW can be a cost-effective additive for the sustainable production of biodiesel from Chlorella sp. CKC2.