제주대학교 Repository

Production Study of the Functional Bioactive Compound from Lactobacillus rhamnosus

Metadata Downloads
Author(s)
마오롬니아
Issued Date
2017
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000008190
Abstract
Diacetyl is a high value product that can be significantly used as a food ingredient. Based on the knowledge of involving in diacetyl production, various strategies have been characterized to improve the growth rate and diacetyl production by lactic acid bacteria. The lactic acid bacteria such as Lactobacillus rhamnosus can be used to produce flavour compounds like diacetyl. In this study, diacetyl production of L. rhamnosus GG strain was increased as L. rhamnosus GG was cultured with glucose and pyruvate medium than in MRS media. In order to increase diacetyl production, 20 mM Fe^3^+ was added to the fermentation broth both on MRS and glucose and pyruvate medium at the optimal time. Furthermore, recently a method using surface sterilization Dielectric Barrier Discharge (DBD) air plasma can be considered to make mutagenesis of bacteria. The DBD air plasma method at atmospheric pressure was performed to induce mutation of L. rhamnosus GG. In this study, we treated L. rhamnosus GG using cold atmospheric plasma at Petri dishes with cultured bacterial by a DBD reactor. Sterilization of L. rhamnosus GG for less than 5min was carried out. Thus, a concentration of diacetyl was increased by using the plasma treatment mutagenesis at 150V at 2min on L. rhamnosus GG strain.
Affiliation
제주대학교 일반대학원
Department
대학원 차세대융복합과학기술협동과정
Advisor
이동선
Awarded Date
2017. 8
Table Of Contents
CONTENTS i
LIST OF ABBREVIATIONS iii
LIST OF FIGURES iv
ABSTRACT 1
INTRODUCTION 2
MATERIALS AND METHODS 4
1. Microorganism 4
2. Maintaining cultured cell 4
3. Inoculum and fermentation media 4
3.1. MRS medium 4
3.2. Glucose and pyruvate medium 6
3.3. Iron (III) addition 6
4. Diacetyl assay 6
5. Method of dielectric barrier discharge 7
RESULTS 11
1. Optimization of incubation period, temperature and diacetyl measurement 11
2. Optimization of Fe^3^+addition 13
3. Effect of medium composition and Fe^3^+ on bacteria growth and diacetyl production 15
4. The mutagenesis of L. rhamnosus GG using plasma treatment by gas pressure dielectic barrierd discharge (DBD) 17
5. Effect of dielectic barrierd discharge (DBD) plasma treatment on bacteria growth and diacetyl production 19
6. Processing of diacetyl assay on L. rhamnosus GG using plasma mutagenesis 20
7. Diacetyl production by addition of Fe^3^+ after plasma treatment 23
DISCUSSION 25
CONCLUSION 27
REFERENCES 28
ACKNOWLEDGEMENTS 34
Degree
Master
Publisher
제주대학교 일반대학원
Citation
마오롬니아. (2017). Production Study of the Functional Bioactive Compound from Lactobacillus rhamnosus
Type
Dissertation
Appears in Collections:
General Graduate School > Interdisciplinary Graduate Program in Advanced Convergence Technology and Science
Authorize & License
  • AuthorizeOpen
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.