붓순나무 추출물의 세포독성 및 항산화 효과

Abstract

This study was implemented to investigate the cytotoxicity and antioxidant functionality of Illicium anisatum L natural product. The purpose of this study is to provide the basic data for developing how to utilize Illicium anisatum L native to Jeju Island, South Korea and natural product-derived antioxidant. As a result of mycete hyphal growth experience, it was found that the higher the concentration of Illicium anisatum L hot water extract, the more the hyphal growth was inhibited. With regards to ABTS radical scavenging ability, it was found that the leaves showed over 50% antioxidative effect in n-butanol fraction at 12.5μg/㎖ concentration; and branches, antioxidative effect higher than BHT in 70% etanol, chloroform, ethyl acetate, n-butanol factions. In regards to DPPH radical scavinging ability, the leaves were found to have over 50% antioxidative effect in ethyl acetate fraction at 100μg/㎖ concentration; and branches, oxidative effect higher than BHT in n-butanol, and ethyl acetate fractions at 12.5μg/㎖ concentration. As a result of phenol examination, the leaves showed the highest amount of chloroform, followed by 70% ethanol, and ethyl acetate in order. In the branch extract, n-butanol and ethyl acetate factions were found in large amount each. Both leaves and branches showed high amounts of flavonoids in n-butanol and ethyl acetate fractions. Cytotoxicity examination found no cytotoxicity in HaCat cell of leaf and branch hot water extracts at 250㎍/㎖ concentration. The 70% etanol extract was found to have no cytotoxicity in leaves at 250㎍/㎖ concentration and branch at 31.25㎍/㎖. In raw 267.7 cell, the leaf and branch hot water extracts showed no toxicity at 250㎍/㎖. The 70% etanol extract had no cytotoxicity in leaves at 125㎍/㎖, and branches at 62.5㎍/㎖. Based on the findings above, it seems that some specific ingredients of phenol and flavonoids contained in Illicium anisatum L ethyl acetate fraction help affect mycete growth and antioxidative ability. Moreover, Illicium anisatum L cytotoxicity was not found in HaCat cell and Raw 267.7 cell under a certain concentration level, indicating the possibility to be utilized as a natural antioxidant to replace the synthesized antioxidant, BHT.