노박덩굴 추출분획물의 항산화 및 미백활성 평가

Abstract

This study was implemented to investigate Antioxidant and Tyrosinase Inhibitory Activities of Celastrus orbiculatus Thunb. climbing stem natural product. The purpose of this study is to provided the basic data for developing how to utilized Celastrus orbiculatus Thunb. native to Gotjawal Jeju Island, South Korea and natural product-derived antioxidant. As a result of total phenolic contents are showed the highest amount of ethyl acetate(320.51 ± 4.25mg/g), followed by n-butanol, chloroform, 80% ethanol, n-hexane and aqueous from Celastrus orbiculatus Thunb.. The climing stems showed low amounts of flavonoids in all of fraction from extract and a extract. In antioxidation activity ABTS, RC50 which was necessary concentration for expressing the 50% inhibition activity. n-butanol fractions that value is (4.62 ± 0.20㎍/㎖) was the highest in ABTS. ethyl acetate(5.69 ± 0.25㎍/㎖) was the second highest value after n-butanol. Especially, antioxidative effect higher than BHT in n-butanol and Ethyl acetate fractions over 12.5μg/㎖ concentration. In antioxidation activity DPPH, RC50 which was necessary concentration for expressing the 50% inhibition activity. n-butanol fractions that value is (20.98 ± 0.51㎍/㎖) was the highest in DPPH. ethyl acetate(29.24 ± 1.05㎍/㎖) was the second highest value after n-butanol. Especially, antioxidative effect higher than BHT in n-butanol and Ethyl acetate fractions over 6.25μg/㎖ concentration. n-butanol fraction showed the highest DPPH and ABTS radical scavenging activity. ethyl acetate was the second highest value after n-butanol.

Inhibition of Tyrosinase activity, IC50 which was necessary concentration for expressing the 50% inhibition activity. ethyl acetate fractions that value is (78.06 ± 18.16㎍/㎖) was the highest in inhibition of tyrosinase activity. chloroform(79.67 ± 13.57㎍/㎖) was the second highest value after ethyl acetate. Especially, antioxidative effect higher than arbutin in ethyl acetate and chloroform fractions over 25μg/㎖ concentration. Correlation coefficient between active components and their biological activities of climbing stem extract and fractions were found that there are some high correlation of Phenol, ABTS, DPPH, and Tyrosinase. Based on the findings above, Celastrus orbiculatus Thunb. has the possibility to be utilized as a natural antioxidant or cosmetic products to replace the synthesized antioxidant, BHT.