제주도 동부지역 버섯의 형태적 동정과 DNA 염기서열 분석을 통한 동정 비교

Abstract

This research is conducted to purpose securing biological genetic resources and supplementing basic data of species diversity researches, suggesting necessity of identification by DNA sequence analysis in species classification. The research were proceeded at four areas Muryeongarioreum, Sillyecheon-Iseungak forest path, Gyoraegotjawal, Geomunoreum suited to mushroom's occurrence in 2016. The collected mushrooms are classified as 15 orders 56 families 149 genera 317 species. By regional groups, 12 orders 31 families 63 genera 130 species are the most collected at Sillyecheon-Iseungak forest path, 13 orders 43 families 83 genera 122 species at Muryeongarioreum, 13 orders 38 families 72 genera 98 species at Gyoraegotjawal, and 11 orders 29 families 61 genera 91 species are the least collected at Geomunoreum. By monthly, 14 orders 44 families 107 genera 199 species are the most collected in July, 13 orders 33 families 72 genera 119 species in August, 8 orders 30 families 54 genera 71 species in Jun, 9 orders 28 families 44 genera 53 species in May, and 6 orders 17 families 25 genera 28 species are the least collected in September. The collected mushrooms are classified as symbionts 145 species, saprobes 166 species, parasites 6 species. And DNAs of 129 species were extracted among 172 species saprobes and parasites incubated at MCM, and DNAs of 25 species were identified from extracted DNAs. In them, it was classified that 1 species re-identified by DNA sequence analysis after they were classified as sp. because of difficulty in morphological identifying, 4 species re-identified after they were identified as allied species, and 20 species matched the results of morphological identification. This research is considered to be useful to basic data of mushroom research in Jeju island. And because the latest method of identifying species is changed from identifying only morphological characters to combining DNA sequence analysis as well as morphological characters, it suggests a necessity of recently changing research approach based on the research result.