스타푸르트 가지 추출물의 항산화, 항염 및 항균 활성 성분 연구

Abstract

In other to develop bioactive ingredients from subtropical resources, we screened Averrhoa carambola branches collected in Jeju Island. The prepared 70% ethanol extract was successively partitioned into n-Hex, EtOAc, n-BuOH and H2O fractions. Upon biological screenings, EtOAc and n-BuOH fractions exhibited potent anti-oxidative, anti-inflammatory and anti-bacterial activities. Phytochemical investigation of the extract from A. carambola branches resulted in isolation of four constituents; epi-catechin (1), daucosterol (2), phloretin 3′-C-(4-O-β-D-glucopyranosyl)-β-D-fucopyranosyl-6′-O-α-L-arabinofuranoside (3) and carambolaside J (4). The chemical structures of isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. As far as we know, compounds 1 and 3 were isolated for the first time from this plant and compound 3 was identified as a novel compound in nature. Quantitative analysis was performed by HPLC. The contents of compounds 1, 3 and 4 were confirmed in extract (2.19 mg/g, 7.75 mg/g, 3.00 mg/g), EtOAc (35.18 mg/g, 4.61 mg/g, 4.85 mg/g) and n-BuOH (2.16 mg/g, 29.38 mg/g, 14.65 mg/g) fractions, respectively. On the anti-oxidation tests, the compound 1 (SC50 38.7 μM) showed stronger DPPH radical scavenging activity than positive control (ascorbic acid, SC_50 43.5 μM). Also, compounds 1 (SC_50 12.0 μM), 3 (SC_50 20.9 μM), and 4 (SC_50 34.2 μM) exhibited potent ABTS^+ radical scavenging activities compared to ascorbic acid (SC_50 31.8 μM). For the anti-inflammation studies using RAW264.7 cells, compounds 1, 3 and 4 inhibited the production of NO effectively without causing cell toxicity. Furthermore, the compound 3 inhibited the generation of iNOS protein. Based on these results, it was suggested that extract and isolated compounds from A. carambola branches could be potentially applicable as pharmaceutical and/or cosmetic ingredients.