물사과 가지, 풍란 캘러스 추출물의 항산화 및 항염 활성 성분 연구

Abstract

In this study, we investigated identification of anti-oxidative and anti-inflammatory constituents from Syzygium aqueum Burm.f. Alston branches and Neofinetia falcata Thunb. Hu callus. The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. Four constituents were isolated from the extract of S. aqueum branches; 3-O-methylellagic acid 3'-O-α-L-rhamnopyranoside (1), 3-O-methylellagic acid 3'-O-α-L-3''-O-acethylrhamnopyranoside (2), pinocembrin (3) and desmethoxymatteucinol (4). As far as we know, compounds 1, 2 and 4 were isolated for the first time from this plant. For the anti-oxidative activities, the EtOAc and n-BuOH layers showed potent DPPH, ABTS^+ radical scavenging activities. Also, among the isolated compounds, compound 1 exhibited stronger ABTS^+ radical scavenging activity than ascorbic acid (positive control). In an anti-oxidative activity assay using HaCaT keratinocyte, the compounds 3 and 4 protected the cell against oxidative stress by H_2O_2. For the anti-inflammation studies using RAW264.7 macrophage, the EtOAc layer reduced expression of iNOS and inhibited the production of NO, PGE_2 and pro-inflammatory cytokines (IL-1β, IL-6). Moreover, compounds 1, 3 and 4 inhibited the production of NO, effectively. Three phytochemicals were isolated from the extract of N. falcata callus; kaempferol 3-O-robinobioside (5), β-sitosterol (6) and daucosterol (7). As far as we know, all of the compounds were isolated for the first time from this plant callus. The EtOAc layer and compound 5 was examined for their anti-oxidation activities by DPPH and ABTS^+ radical assays. As a result, EtOAc layer and compound 5 showed anti-oxidative activities. For the anti-inflammation studies, the EtOAc layer inhibited the production of NO, PGE_2 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), effectively. Based on these results, it was suggested that extract and isolated compounds from S. aqueum branches and N. falcata callus could be potentially applicable as anti-oxidative and/or anti-inflammatory ingredients.