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Comparative Molecular Characterization of Tumor Necrosis Factor alpha (TNFα) and Lipopolysaccharide Induced TNFα Factor (LITAF) genes from mullet Liza haematocheila

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Abstract
Mullets are commercially important fishes throughout the world. Even though, production from mullet farming is increasing in recent years, mass mortality associated with pathogenic infections causes heavy economic loss. For the success of Korean mullet aquaculture, comprehensive understanding of mullet immune system is essential for better health management. TNF alpha and LITAF genes are important immune genes studied from wide range of aquatic organism for better understanding. This study showed that TNF alpha and LITAF genes are expressed ubiquitously in various tissues of mullet and upregulated with pathogenic challenge. LITAF genes were showed to be participating in apoptosis. All three mullet LITAF genes induce capase-3 activity. These LITAFs also induce TNF alpha expression. These study showed that TNF alpha and LITAF are critical immune genes could be targeted for better disease control in mullet.
Author(s)
Amirthalingam, Pavithiran
Issued Date
2018
Awarded Date
2018. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000008583
Affiliation
제주대학교 일반대학원
Department
대학원 해양생명과학과
Table Of Contents
Acknowledgement iii
Abstract v
Table of Content vi
List of Figures viii
List of Tables x
1. Introduction 1
1.1. Global mullet fishery 1
1.2. Korean mullet fishery and aquaculture2
1.3. Lipopolysaccharide induced tumor necrosis factor α factor 4
1.4 . Tumor Necrosis Factor Alpha 5
1.5. Aim of the study 6
2. Methodology 7
2.1. Transcriptome library construction7
2.2. Experimental fish and tissue collection 7
2.3. RNA extraction and cDNA synthesis 8
2.4. Expression analysis by quantitative real-time PCR (qRT-PCR) 8
2.5. Construction of the recombinant vector 9
2.6. Subcellular localization analysis . 9
2.7. Hoechst staining10
2.8. Caspase-3 activity 10
2.9. TNFα mRNA expression analysis10
3. Results and Discussion 11
3.1. In Silico analysis 11
3.2. Multiple sequence alignment 14
3.3. Phylogenetic analysis 17
3.4. Tissue specific expression 20
3.5. Challenge experiment 24
3.6. Subcellular localization 32
3.7. Hoechst staining 33
3.8. Caspase-3 activity 35
3.9.TNFα mRNA expression analysis 36
4. Conclusion 37
5. References 38
6. Appendix42
Degree
Master
Publisher
제주대학교 일반대학원
Citation
Amirthalingam, Pavithiran. (2018). Comparative Molecular Characterization of Tumor Necrosis Factor alpha (TNFα) and Lipopolysaccharide Induced TNFα Factor (LITAF) genes from mullet Liza haematocheila
Appears in Collections:
General Graduate School > Marine Life Sciences
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