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Juniperus chinensis extract induces apoptosis via ROS generation in human pancreatic cancer cell lines

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Abstract
Pancreatic cancer is one of the dreadful tumors. Less symptoms or signs at early stage cause over a half of patients has distant metastases, which leads resistance to cancer therapies. In this study, we found Juniperus chinensis extract (JCX) inhibited the cell viability and migration activity of PANC-1 and SNU-213 in a dose-dependent manner. JCX increased capase-3 activation, and reactive oxygen species (ROS) generation. N-acetylcysteine (NAC) treatment blocked anti-viability activity of JCX and JCX -induced ROS generation. In
addition, JCX downregulated the levels of p-FAK/FAK and p-ERK/ERK. Altogether, these results indicated that JCX induced apoptosis in human pancreatic cancer cell lines via ROS generation and downregulation of FAK/ERK signaling and activation of caspase-3. We suggest the JCX-derived compounds could be an alternative medicine candidate for pancreatic cancer treatment.
Author(s)
고보람
Issued Date
2018
Awarded Date
2018. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000008640
Alternative Author(s)
Go, Bo Ram
Affiliation
제주대학교 일반대학원
Department
대학원 생명공학과
Table Of Contents
LIST OF FIGURES III
LIST OF TABLES V
ABSTRACT VI
1. Introduction1
2. Materials and Methods 3
2.1. Sample preparation and antibodies 3
2.2. Cell culture 3
2.3. Cell viability assay 3
2.4. Scratch wound healing assay 4
2.5. Western blot analysis 4
2.6. Flow cytometry analysis 5
2.7. GC-MS MS analysis 5
2.8. Statistical analysis 6
3. Results7
3.1. JCX inhibits cell viability in human pancreatic cancer cell lines. 7
3.2. JCX inhibits cell migration in human pancreatic cancer cells lines. 10
3.3. JCX downregulates the tyrosine-phosphorylated
proteins in human pancreatic cancer cell lines. 12
3.4. JCX induces apoptosis through activation of caspase-3
in human pancreatic cancer cells lines. 15
3.5. JCX induces ROS generation in PANC-1 and SNU-213 18
3.6. GC-MS MS analysis 21
4. Discussion 25
REFERENCES27
ACKNOWLEDGMENT
Degree
Master
Publisher
제주대학교 일반대학원
Citation
고보람. (2018). Juniperus chinensis extract induces apoptosis via ROS generation in human pancreatic cancer cell lines
Appears in Collections:
General Graduate School > Medicine
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