개 혈액시료에서 바베시아 진단을 위한 간이 항체진단 키트와 실시간 qPCR의 평가

Abstract

Babesia is a protozoal disease caused by ticks in cattle, sheep, pigs, horses, dogs and humans. Canine babesiosis is caused by Babesia gibsoni, B. canis supsp., B. microti, B. equi, B. conradae and B. vitalii. Canine babesiosis is morphologically divided into small and large babesia. B. gibsoni which is small babesia and mediated by Haemaphysalis longicornis and Haemaphysalis bispinosa, occurs mainly in South Korea and throughout Asia. B. gibsoni is generally infected by the mites bite and are also infected by transfusions from infected dogs and through placental infection. In recent years, outbreak of babesiosis is increasing in all country of South Korea as well as Jeju Island. In this study, I have surveyed the prevalence of canine babesiosis in Jeju, and estimated the sensitivity and specificity of canine one step babesia antibody kit based on the PCR results and also verified the utility of qPCR in local clinics. From May 2018 to November 2018, 211 dogs were tested by CBC, canine babesia Ab kit(Bionote, Inc, Korea), CRP, PCR and qPCR. Canine babesia Ab kit showed 74 dogs were positive and 137 dogs were negative. In PCR test, 99 dogs of them were positive (46.9%). In the results of canine babesia Ab kit based on the PCR, the sensitivity and specificity of babesia are 62.6% and 89.3%, respectively. The results of qPCR were the same as those of general PCR. This study have shown that PCR and CRP test be performed at the beginning of Barbecia infection and PCR and babesia antibody test kit at the time of infection showing clinical symptoms. Since the antibody is persistent in the absence of the antigen after the treatment, the antibody test kit should be carefully considered after treatment. And easy, fast and accurate qPCR can be useful method for diagnosis and prognosis of babesiosis in clinics.