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성장기 흰쥐에서 높은 농도의 혈중코티졸이 해마 신경의 연접가소성 및 인지행동기능에 미치는 영향

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Alternative Title
The effects of high blood cortisol on synaptic plasticity of hippocampal neurons and cognitive behaviors
Abstract
The high level of blood cortisol is the key factor to identify major depressive disorder (MDD) which is mediated with the abnormal modulation of brain-derived neurotrophic factor (BNDF) in mammalian brains. However, it is not well known if and how the elevation of cortisol level during prenatal period affects brain functions and induces psychiatric disorders such as MDD after birth. For this issue, we constantly elevated the cortisol level of prenatal rats by injecting corticosterone (20 mg/kg) to maternal rats every day for 21 consecutive pregnant days until delivery. This procedure critically elevated cortisol level in both maternal and postnatal pups. After delivery, pups were bred with their mother rat and isolated from their mother in postnatal 21st day for behavioral tests. Behavioral tests to observe cortisol effects in brain functions were performed by hiring a forced swim test (FST), Morris water maze test (MWT) and open field moving test (OFT). Pups delivered from corticosterone-injected maternal rats (Corti.Pup) showed significantly different behavioral patterns, compared with normal pups (Normal, saline-injected). In FST performed for 5 min, immobility time of Corti.Pup was critically shorter than that of Normal, showing anxiety-mediated hyperactivity. In addition, Corti.Pup were confirmed to have the critical impairment of learning and memory functions in MWT as they needed longer time to figure out where a hidden platform located. These behavioral patterns of Corti.Pup seemed to be correlated with those showing in attention deficit hyperactivity disorder (ADHD) patients. To clarify the impairment of memory function, I also tried electrophysiological experiments to observe neuronal characteristics and memory functions in a cellular level. Using hippocampal slices (p14~18), patch-clamp recordings for observing the long-term potentiation (LTP) patterns and membrane excitability in CA1 neurons were performed. In results, Corti.pup showed higher excitable properties of CA1 membranes and incomplete potentiation in LTP pattern. Therefore, it is possible that cortisol may affect learning and memory functions in developmental brains and consequently behaviors are revealed as the abnormal patterns similar with ADHD.
Author(s)
김혜지
Issued Date
2019
Awarded Date
2019. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000008882
Alternative Author(s)
Kim, Hye Ji
Affiliation
제주대학교 대학원
Department
대학원 의학과
Advisor
정성철
Table Of Contents
Abstract I
목차 III
List of Figures IV
서론 . 1
연구 방법 . 6
결과 12
1) 동물모델 사이의 행동학적 특징 비교 12
2) 성장기 흰쥐 hippocampal CA1 neuron의 전기생리학적 특성 비교 22
3) LTP 차이에 따른 세포 단위의 기억 기능의 이해 . 30
고찰 . 35
참고문헌 39
Degree
Master
Publisher
제주대학교 대학원
Citation
김혜지. (2019). 성장기 흰쥐에서 높은 농도의 혈중코티졸이 해마 신경의 연접가소성 및 인지행동기능에 미치는 영향
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General Graduate School > Medicine
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