등수국 줄기 추출물의 항산화, 항염 및 미백 활성 성분 연구

Abstract

In this study, we investigated anti-oxidative, anti-inflammatory and whitening constituents from Hydrangea petiolaris stems. Phytochemical investigation of the extract from H. stems resulted in isolation of eight constituents; methyl linoleate (1), β-sitosterol (2), daucosterol (3), oleanolic acid (4), ursolic acid (5), catechin (6), epi-catechin (7) and lyoniside (8). The chemical structures of isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. On the anti-oxidation tests, the compounds 6 (SC50 36.5 μM), 7 (SC50 73.1 μM) and 8 (SC50 61.7) exhibited potent DPPH radical scavenging activity compared to positive control (ascorbic acid, SC50 51.6 μM). Also, compounds 6 (SC50 19.9 μM) and 8 (SC50 24.9 μM) showed stronger ABTS+ radical scavenging activities than positive control (ascorbic acid, SC50 36.2 μM). For the anti-inflammation studies using RAW264.7 macrophage, n-Hex and EtOAc layers inhibited the production of NO effectively without causing cell toxicity. In addition, n-Hex and EtOAc layers reduced expression of iNOS protein and production of PGE2 and pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). B16F10 melanoma cells were used for whitening activity studies. As a result, n-Hex layer inhibited the melanogenesis and intracelluar tyrosinase effectively without causing cell toxicity. Based on these results, it was suggested that extract and isolated compounds from H. petiolaris stems could be potentially applicable as pharmaceutical and/or cosmetic ingredients.