올벚나무 잎 추출물의 항산화 및 항염 활성 성분 연구
- Alternative Title
- Anti-oxidative and Anti-inflammatory Constituents from Extract of Prunus pendula for. ascendens Leaves
- Abstract
- In this study, Prunus pendula for. ascendens (Makino) Ohwi was examined to identify the anti-oxidative and anti-inflammatory constituents. The chemical structures of the isolated compounds were elucidated by analyzing the spectroscopic data including 1H and 13C NMR spectra, as well as the comparison of the data to the literature values.
Five phytochemicals were isolated from EtOAc fraction of P. pendula for. ascendens leaves; ursolic acid (1), prunasin (2), methyl p-coumarate (3), kaempferol (4) and astragalin (5). As far as we know, compounds 1-5 were isolated for the first time from this plant.
The content of the 70% EtOH extract and EtOAc fraction, including compound 5, were analyzed quantitatively using high pressure liquid chromatography (HPLC).
On the anti-oxidation tests, the compound 4 (SC50 100.2 μM) and 5 (SC50 255.3 μM) showed good DPPH radical scavenging activity than positive control (BHT, SC50 247.3 μM). Also, compounds 3 (SC50 158.4 μM), 4 (SC50 53.3 μM) and 5 (SC50 180.3 μM) exhibited potent ABTS+ radical scavenging activities compared to BHT (SC50 34.0 μM).
For the anti-inflammation studies using RAW264.7 macrophage cells stimulated with LPS, EtOAc fraction and compounds 4 and 5 inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), pro-inflammatory cytokines (IL-1β and IL-6), expression of iNOS and COX-2 protein, significantly.
Based on these results, it was suggested that extract and isolated compounds from P. pendula for. ascendens leaves could be potentially applicable as anti-oxidative and/or anti-inflammatory cosmetic ingredients.
- Issued Date
- 2019
- Awarded Date
- 2019. 2
- Type
- Dissertation
- Alternative Author(s)
- Hong, Hye Jin
- Affiliation
- 제주대학교 대학원
- Department
- 대학원 화학과
- Advisor
- 이남호
- Table Of Contents
- List of Tables iv
List of Figures v
List of Abbreviations vii
Abstract ix
Ⅰ. 서론 1
1. 천연물 1
2. 항산화 2
3. 염증 3
Ⅱ. 재료 및 실험 방법 5
1. 시약 및 기기 5
2. 재료 6
3. 추출, 분획 및 활성 성분 분리 7
1) 올벚나무 잎의 추출 및 용매 분획 7
2) Ethyl acetate 분획물의 활성 성분 분리 8
4. 생리 활성 평가 9
1) 항산화 9
(1) 총 폴리페놀 함량 측정 9
(2) 총 플라보노이드 함량 측정 10
(3) DPPH radical 소거 활성 측정 11
(4) ABTS+ radical 소거 활성 측정 12
2) 항염 13
(1) RAW264.7 세포 배양 13
(2) Nitric oxide (NO) 생성 억제 활성 측정 13
(3) 세포 독성 평가(MTT assay) 13
(4) PGE2 및 전염증성 cytokine 생성 억제 활성 측정 14
(5) Western blot analysis 14
5. HPLC analysis 16
Ⅲ. 결과 및 고찰 17
1. 분리된 화합물의 구조 분석 17
1) Compound 1의 구조 동정 17
2) Compound 2의 구조 동정 20
3) Compound 3의 구조 동정 23
4) Compound 4, 5의 구조 동정 26
2. HPLC 분석 결과 31
3. 올벚나무 잎 추출물 및 분획물의 활성 실험 결과 32
1) 항산화 32
(1) 총 폴리페놀 함량 측정 32
(2) 총 플라보노이드 함량 측정 34
(3) DPPH radical 소거 활성 측정 35
(4) ABTS+ radical 소거 활성 측정 36
2) 항염 37
(1) Nitric oxide (NO) 생성 억제 활성 측정 37
(2) PGE2 생성 억제 활성 측정 39
(3) 전염증성 cytokine 생성 억제 활성 측정 40
(4) iNOS, COX-2 단백질 발현 억제 활성 측정 42
4. 분리된 화합물의 활성 실험 결과 43
1) 항산화 43
(1) DPPH radical 소거 활성 측정 43
(2) ABTS+ radical 소거 활성 측정 45
2) 항염 47
(1) Nitric oxide (NO) 생성 억제 활성 측정 47
Ⅳ. 요약 및 결론 48
Ⅴ. 참고문헌 50
- Degree
- Master
- Publisher
- 제주대학교 대학원
- Citation
- 홍혜진. (2019). 올벚나무 잎 추출물의 항산화 및 항염 활성 성분 연구
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- General Graduate School > Chemistry
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