간이진단법을 활용한 개 매개체 전염병의 진단

Abstract

Vector-borne diseases are caused by viruses, bacteria, protozoa, and parasites that are transmitted by arthropods such as mosquitoes, ticks, fleas and the like. This causes clinically significant diseases such as heartworm disease through mosquitoes and anaplasmosis ehrlichiosis, Lyme borreliosis, babesiosis through ticks. Globally, the incidence of vector-borne disease is also affected by accelerated global warming, increased temperature and precipitation, increased arthropod survival rate, laying rate, activity rate and increased arthropod populations. This vector-borne disease has confirmed that the rate of incidence has not been well researched in Korea, especially in Jeju Island. In this study, I've investigated prevalence of vector-borne diseases in dogs and estimate the sensitivity and specificity of the new developed Cani-V4 Ab test kit(BioNote). In total 281 dogs(stray dogs; 205, patient dogs; 76), blood samples were collected from jugular vein from August 2018 to March 2019. The blood sampleswere performed with general blood test (CBC), blood smear test, acute phase reaction protein (CRP), and Bionote cani-v4 kit. The genes were isolated and the real time PCR was performed together. In 281 blood samples, only positive of babesia, heartworm, anaplasma, borellia and ehrlichia are 81(28.8%), 41(14.6%), 4(1.4%), 0, 0, respectively. Coinfected dogs of babesia and heartworm, babesia and anaplasma, babesia and borellia, babesia and ehrlichia, babesia and anaplasma and ehrlichia, babesia and anaplasma and borellia and ehrlichia are 11(3.9%), 22(7.8%), 2(0.7%), 1(0.35%), 1(0.35%), 1(0.35%), respectively. The sensitivity and specificity of Anigen rapid anaplasma Ab test kit are 60.0% and 77.6%. The sensitivity and specificity of Anigen rapid ehrlichia Ab test kit are 15.4% and 100%. The results of antibody and antigen test were inconsistent. Concomitant testing of antibodies and antigens may be necessary in clinical practice.