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Quantitative Assessment of the Reproductive Effort of Tresus keenae (Kuroda and Habae 1950 ) on the South Coast of Korea Using Egg Specific Polyclonal Antibody

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Abstract
Reproductive cycle and reproductive effort of Tresus keenae in Yeosu, South coast of Korea was determined using immune assay techniques combine with the histology. The polyclonal antibody developed against the egg proteins of T. keenae, only detected the egg proteins with a high sensitivity (0.64-10.24µg/ml) was used to quantification of the eggs using indirect ELISA. T. keenae in Yeosu spawned in late autumn (October and November) and ready to spawn female and spawning male observed in late winter (March) while first clam with oogonial mitosis also observed in March. The mean GSI of ready to spawn clam in October was 5.7±2.2 and March was 2.4±0.3. Condition Index (CI) from October to November was declined, however during March CI was not scaled down and potential fecundity of the T. keenae in Yeosu estimated to be varied from 121.92×106 to 22.07×106 (average 53.40×106 ) eggs per clam in October while it was ranged from 69.19×106 to 29.07×106 with average of 44.91×106 eggs per clam in March. The dry weight of siphon and carbohydrate of the siphon declined during the early development of the gametes suggesting that the carbohydrate stored in the siphon exploited for gametogenesis. A noticeable reduction of total carbohydrate in adductor muscle, siphon and body was observed during the spawning period suggesting that the carbohydrate fulfilled the energy demand during the spawning. The egg specific antibody developed in this study is sensitive enough to quantify the reproductive effort of the clam which is difficult to measure using the conventional methods and using developed antibody reproductive effort of T. keenae was successfully determined.
Author(s)
Nainanayaka Pathirannehelage Harshani Deepa Kumari
Issued Date
2019
Awarded Date
2019. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000009118
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
Choi, Kwang Sik
Table Of Contents
Acknowledgment. ⅱ
List of figures. ⅴ
List of tables ⅶ
Abstract. 1
1. Introduction. 2
2. Material and Methods. 7
2.1. Study area and sampling 7
2.2. Histology. 8
2.3.Purification of T. keenae eggs and development of the egg specific antibody 10
2.4. SDS-PAGE and western blotting assay. 11
2.5. Indirect ELISA and Immunofluorescence assay. 12
2.6. Quantification of T. keenae eggs using indirect ELISA 13
2.7.Tisuue biochemical analysis. 14
3. Results 15
3.1. Annual gametogenesis. 15
3.2. Weight of siphon and adductor muscle. 19
3.3. Biochemical composition of adductor muscle and siphon 20
3.4. Specificity of the antibody 23
3.5. GSI estimated from ELISA. 25
3.6. Potential fecundity estimated from ELISA GSI estimated from ELISA 27
3.7.Condition Index (CI). 27
4. Discussion. 29
4.1. Reproductive cycle of T. keenae in Yeosu. 29
4.2. Energy storage and utilization related to the reproductive cycle. 31
4.3. Specificity of the rabbit anti T. keenae egg IgG 34
4.4. Reproductive effort 35
5.0. Conclusion. 40
6.0. Reference 41
Degree
Master
Publisher
제주대학교 대학원
Citation
Nainanayaka Pathirannehelage Harshani Deepa Kumari. (2019). Quantitative Assessment of the Reproductive Effort of Tresus keenae (Kuroda and Habae 1950 ) on the South Coast of Korea Using Egg Specific Polyclonal Antibody
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General Graduate School > Marine Life Sciences
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