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Sulforaphane decreases telomerase activity and enhances TRAIL-mediated apoptosis by increasing ROS production

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Abstract
Sulforaphane (SFN), a dietary isothiocyanate, is a well known natural product that possesses anti cancer and chemopreventive activities. However, the molecular mechanism of the anti telomerase activity of SFN is not well understood. In this study, we investigated the hypothesis that SFN inhibits cell viability and telomerase activity via downregulation of telomerase reverse transcriptase (hTERT) expression. We suggest that elevated intracellular reactive oxygen species (ROS) levels, due to exposure to SFN, has a critical role in abolishing since pretreatment with NAC, an antioxidant, resulted in the recovery of hTERT expression. SFN also suppressed the phosphorylation of Akt (Ser 473), thereby inhibiting hTERT phosphorylation and this effect was reversed by pretreatment with NAC. Taken together, these data suggest that ROS are essential for the suppression of SFN mediated telomerase activity via transcriptional and posttranslational regulation of hTERT.
Author(s)
Kang, Sang Hyuck
Issued Date
2019
Awarded Date
2019. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000009197
Alternative Author(s)
강상혁
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
Kim, Gi Young
Table Of Contents
Chapter Ⅰ : Sulforaphane decreases viability and telomerase activity in hepatocellular carcinoma Hep3B cells through the reactive oxygen species dependent pathway. 1
Chapter Ⅱ : TRAIL Attenuates Sulforaphane mediated Nrf2 and Sustains ROS Generation, Leading to Apoptosis of TRAIL resistant Human Bladder Cancer Cells. 29
Chapter Ⅲ : Chemopreventive and chemotherapeutic effect of sulforaphane. 70
Degree
Doctor
Publisher
제주대학교 대학원
Citation
Kang, Sang Hyuck. (2019). Sulforaphane decreases telomerase activity and enhances TRAIL-mediated apoptosis by increasing ROS production
Appears in Collections:
General Graduate School > Marine Life Sciences
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