등수국 및 보리밥나무 잎 추출물의 항산화 항염 미백 활성 성분 연구

Abstract

In this study, we investigated anti-oxidative, anti-inflammatory and whitening constituents from leaves of H y d r a n g e a p e t i o l a r i s and Elaeagnus macrophylla . The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. Twelve constituents were isolated from the extract of Hydrangea petiolaris leaves; epi -catechin (1), hyperin (2), quercitrin (3), afzelin (4), 1O p -coumaroyl- -D-glucopyranoside ( β 5), asiatic acid (6), corosolic acid (7), ethyl linoleate (8), ethyl linolenate (9), phytol (10), 1-linoleoyl glycerol (11) and 1-linolenoyl glycerol (12). On the anti-oxidative tests, the extract, EtOAc and n -BuOH fractions and compounds 1-4 showed potent free radical scavenging activities. Also, for the cellular protective effects on HaCaT keratinocytes damaged by H2O2, the n -Hex, EtOAc fractions and compound 1, 3 indicated protective effects against oxidative stress. In the anti-inflammatory tests using RAW 264.7 cells, the n -Hex and EtOAc fractions inhibited the production of NO without causing cell toxicity. In addition, the n -Hex and EtOAc fractions reduced expression of iNOS protein and production of pro-inflammatory cytokines (TNF- , α IL-6, IL-1 ). For the anti-melanogenesis tests using B16F10 melanoma β cells, the n -Hex fraction exhibited potent inhibitory activities of melanin formation without causing cell toxicity. Five phytochemicals were isolated from the extract of Elaeagnus macrophylla leaves; methyl linolenate (1), -tocopherol ( α 2), -sitosterol β (3), -amyrin α (4), β-amyrin (5). In the anti-melanogenesis tests using B16F10 melanoma cells, the n -Hex fraction showed anti-melanogenesis activity without causing cell toxicity. Based on these results, it was suggested that the extracts and isolated compounds from H y d r a n g e a p e t i o l a r i s and E l a e a g n u s m a c r o p h y l l a leaves could be potentially applicable as natural source for pharmaceutical and/or cosmetic ingredients.