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Biological characteristics of the chemosymbiotic lucinid clam Pillucina pisidium inhabiting the lagoonal seagrass bed of the subtropical island

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Abstract
Chemosymbiotic microbes have been identified in the intertidal zones and the deep-sea hy drothermal vents where the environments are enriched with reduced sulfur. Recently, several studies have reported presence of clams in the sediment of shallow subtidal seagrass, harboring chemosynthetic microbes as symbionts. In this study, we investigated an association of chem osynthetic bacteria with a lucinid bivalve Pillucina pisidium occurring in subtidal seagrass bed in a lagoon on the east coast. A total of 360 clams were collected from Zostera marina bed for the analysis. P. pisidium was a small clam with a mean shell length of 7.0 mm and tissue wet weight of 26.2 mg. Histology revealed that P. pisidium has unusually thick gills which at tributed to 16.5% of the total tissue weight. Histology also revealed that the digestive gland and stomach were reduced compared to other bivalves, suggesting that P. pisidium may have another source of food except the filtered food particles from the ambient environment. Scan ning electron microscopy showed symbiotic bacteria in the gills. The results of stable isotope ratio analysis suggest that significantly depleted carbon and nitrogen indicate that bacterial fixation of carbon and nitrogen occurs in the gills. Next Generation Sequencing revealed that thiotrophic and diazotrophic bacteria were dominant gill filaments
Author(s)
Shin, Jong Seop
Issued Date
2020
Awarded Date
2020. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000009363
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
Choi, Kwang Sik
Table Of Contents
1. INTRODUCTION . 1
2. MATERIALS AND METHODS. 4
2.1. Study area and sample collection. 4
2.2. Histological preperation. 6
2.3. Scanning electron microscopy . 6
2.4. Stable isotope ratio analysis. 6
2.5. Endosymbiosis community analysis 7
2.5.1 Genomic DNA isolation 7
2.5.2. Next generation sequencing 7
3. RESULTS 9
3.1. Biometry 9
3.2. Light microscopy . 9
3.3. Ultrastructure of internal organs 9
3.4. Origin of organic matters. 12
3.5. Bacterial community analysis 12
4. DISCUSSION 15
4.1. Study area. 15
4.2. Acquisition of symbionts. 16
4.3. Bacteria-host interactions. 17
REFERENCES. 20
ACKNOWLEDGEMENT . 27
Degree
Master
Publisher
제주대학교 대학원
Citation
Shin, Jong Seop. (2020). Biological characteristics of the chemosymbiotic lucinid clam Pillucina pisidium inhabiting the lagoonal seagrass bed of the subtropical island
Appears in Collections:
General Graduate School > Marine Life Sciences
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