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Evaluation of krill meal supplementation on growth, feed utilization, intestinal morphology, innate immunity and disease resistance of olive flounder (Paralichthys olivaceus)

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Abstract
The supplemental effect of krill meal into a low fish meal (FM) diet was evaluated for olive flounder (Paralichthys olivaceus). A 56% FM-based diet was regarded as a high FM inclusion diet (HFM) and a 28% FM inclusion was considered a low-FM diet (LFM). Four other diets were prepared by supplementing 3, 6, 9 and 12% krill meal into the LFM diet (designated as KM3, KM6, KM9 and KM12, respectively). Quadruplicate groups of fish were fed one of the diets for 12 weeks. The growth performance and feed utilization were improved by the dietary krill meal supplementation. Digestibility of dietary protein and dry matter was increased by the KM3-9 diets. Hematocrit and hemoglobin were increased by krill meal supplementation. The innate immunity assessed by antiprotease, lysozyme, GPx, SOD and Ig and the condition factor of fish were significantly increased by the KM3-9 diets. Moreover, goblet cell counts, villi length and fillet yield of fish were significantly improved by all the krill meal-containing diets (KM3-12). A 25-day long challenge test with the Edwardsiella tarda pathogen showed that the cumulative mortality was higher in fish fed the LFM diet than in fish fed either the HFM or krill meal-supplemented diets. The results indicate that dietary krill meal supplementation in a LFM diet can increase growth performance and feed utilization efficiency, diet digestibility, intestinal development and functions, innate immunity and disease resistance of olive flounder. The recommended level of krill meal inclusion seems to be between 6-9%, when FM feed inclusion is low.
본 연구에서는 넙치(Paralichthys olivaceus) 저어분 사료 내 크릴의 첨가효과를 평가하였다. 대조사료는 총 2가지로, 사료 내 어분의 함량이 56%인 HFM와 28%인 저어분 대조구(LFM)로 조성되었다. 실험사료는 총 4가지로, 크릴밀은 LFM 사료 내 어분을 각각 3, 6, 9, 12% (KM3, KM6, KM9, KM12) 대체하도록 설정되었다. 사육실험은 4 반복으로, 총 12 주간 진행되었다. 사육실험 결과, 사료 내 크릴의 첨가는 넙치의 성장과 사료효율을 증진시키는 것으로 나타났다. 넙치의 단백질과 건물 소화율은 크릴 3-9% 첨가구(KM3-9%)가 대조구에 비해 증가하는 것으로 나타났다. 헤모글로빈과 hematocrit 은 크릴 첨가구가 모두 증가한 것으로 나타났다. 비특이적 면역 지표인 anti-protease, lysozyme, GPx, SOD, Ig 의 활성은 KM3, 6, 9 실험구가 대조구에 비해 유의적으로 높았다. 실험어 장 내 goblet cell의 수, 융모길이, 어체의 가식부비율 또한 모든 실험구(KM3-12)가 대조구에 비해 증가하는 것으로 나타났다. Edwardsiella tarda 병원체를 이용하여 25 일간 진행된 공격실험 결과, 누적폐사율은 LFM 구가 HFM 구와 크릴첨가구에 비해 높게 나타났다. 본 연구에서 저어분 사료 내 크릴의 첨가는 넙치의 성장, 사료효율, 소화율, 장의 발달과 기능, 비특이적 면역반응, 질병저항성을 향상시키는 것으로 나타났다. 결론적으로, 저어분 사료 내 크릴의 적정 첨가함량은 3 9%라고 판단된다.
Author(s)
W M Kasun Tharaka Jayathilaka
Issued Date
2020
Awarded Date
2020. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000009376
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
Lee, Kyeong Jun
Table Of Contents
요약문 iii
ABSTRACT iv
LIST OF TABLES v
LIST OF FIGURES vi
1. INTRODUCTION 1
1.1 Replacement fish meal in aqua-feed 1
1.2 Krill meal 2
1.3 Potential use of krill in aquaculture . 2
1.4 Olive flounder 3
2. MATERIALS AND METHODS
2.1 Experimental diets. 4
2.2 Feeding trial and sample collection. 5
2.3 Determination of apparent digestibility coefficients. 7
2.4 Analyses. 9
2.5 Morphology of body and intestine 10
2.6 Expression levels of liver IGF-I mRNA. 11
2.7 Challenge test 12
2.8 Statistical analysis 13
3. RESULTS 14
4. DISCUSSION. 30
5. CONCLUSIONS 36
6. REFERENCES 37
7. ACKNOWLEDGEMENT 47
Degree
Master
Publisher
제주대학교 대학원
Citation
W M Kasun Tharaka Jayathilaka. (2020). Evaluation of krill meal supplementation on growth, feed utilization, intestinal morphology, innate immunity and disease resistance of olive flounder (Paralichthys olivaceus)
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General Graduate School > Marine Life Sciences
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