생달나무 잎 추출물의 항산화, 항염 및 항균 활성 성분 연구

Abstract

In this study, we investigated identification of anti-oxidative, anti-inflammatory and anti-bacterial constituents from Cinnamomum yabunikkei leaves. Five phytochemicals were isolated from the extract of C. yabunikkei leaves; (4S,5R)-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone (1), methoxy-(3,5-dimethoxy-4-hydroxyphenyl)ethanediol (2), afzelin (3), nicotiflorine (4) and narcissin (5). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. As far as we know, compounds 1-5 were isolated for the first time from this plant. On the anti-oxidative tests, the ethyl acetate (EtOAc) fraction showed potent free radical scavenging activities. In the anti-inflammatory tests using RAW264.7 cells, the EtOAc fraction inhibited the production of nitric oxide (NO) without causing cell toxicity. In addition, the EtOAc fraction reduced expression of iNOS protein and production of pro-inflammatory cytokines (IL-1β, TNF-α). Also compound 1, 3, 4 and 5 inhibited the production of NO effectively without causing cell toxicity. Furthermore, compound 1 reduced expression of iNOS protein and exhibited potent inhibitory activities of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α). Upon the anti-bacterial tests using Staphylococcus epidermidis (CCARM 3709, 3710, 3711) and Cutibacterium acnes (CCARM 0081, 9010), EtOAc fraction showed strong activities. Based on these results, it was suggested that the extract and isolated compounds from C. yabunikkei leaves could be potentially applicable as natural source for pharmaceutical and/or cosmetic ingredients.