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생달나무 잎 추출물의 항산화, 항염 및 항균 활성 성분 연구

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Alternative Title
anti-inflammatory and anti-bacterial constituents from the extract of cinnamomum yabunikkei leaves
Abstract
In this study, we investigated identification of anti-oxidative, anti-inflammatory and anti-bacterial constituents from Cinnamomum yabunikkei leaves. Five phytochemicals were isolated from the extract of C. yabunikkei leaves; (4S,5R)-4-hydroxy-5-isopropyl-2-methylcyclohex-2-enone (1), methoxy-(3,5-dimethoxy-4-hydroxyphenyl)ethanediol (2), afzelin (3), nicotiflorine (4) and narcissin (5). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. As far as we know, compounds 1-5 were isolated for the first time from this plant. On the anti-oxidative tests, the ethyl acetate (EtOAc) fraction showed potent free radical scavenging activities. In the anti-inflammatory tests using RAW264.7 cells, the EtOAc fraction inhibited the production of nitric oxide (NO) without causing cell toxicity. In addition, the EtOAc fraction reduced expression of iNOS protein and production of pro-inflammatory cytokines (IL-1β, TNF-α). Also compound 1, 3, 4 and 5 inhibited the production of NO effectively without causing cell toxicity. Furthermore, compound 1 reduced expression of iNOS protein and exhibited potent inhibitory activities of pro-inflammatory cytokines (IL-6, IL-1β, TNF-α). Upon the anti-bacterial tests using Staphylococcus epidermidis (CCARM 3709, 3710, 3711) and Cutibacterium acnes (CCARM 0081, 9010), EtOAc fraction showed strong activities. Based on these results, it was suggested that the extract and isolated compounds from C. yabunikkei leaves could be potentially applicable as natural source for pharmaceutical and/or cosmetic ingredients.
Author(s)
김소희
Issued Date
2020
Awarded Date
2020. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000009542
Alternative Author(s)
Kim, So Hee
Affiliation
제주대학교 대학원
Department
대학원 화학과
Advisor
이남호
Table Of Contents
List of Tables ⅳ
List of Figures ⅴ
List of Abbreviations ⅶ
Abstract ⅸ
I. 서론 1
1. 항산화 2
2. 항염 4
3. 항균 5
4. 생달나무(Cinnamomum yabunikkei) 7
II. 재료 및 방법 8
1. 시약 및 기기 8
2. 재료 9
3. 생달나무 잎의 추출, 분획 및 활성 성분 분리 10
1) 생달나무 잎의 추출 및 용매 분획 10
2) Ethyl acetate 분획물의 활성 성분 분리 11
(1) MPLC에 의한 분리과정 11
(2) VLC에 의한 분리과정 12
4. 생리 활성 평가 14
1) 항산화 14
(1) 총 폴리페놀 함량 측정 14
(2) 총 플라보노이드 함량 측정 15
(3) DPPH radical 소거 활성 16
(4) ABTS+ radical 소거 활성 17
2) 항염 18
(1) RAW264.7 cell 배양 18
(2) Nitric oxide (NO) 생성 억제 활성 18
(3) Western blot analysis 18
(4) 전염증성 cytokines (IL-6, IL-1β, TNF-α) 생성 억제 활성 19
3) 세포 독성 평가 20
4) 항균 21
(1) 균주 배양 21
(2) Paper disc diffusion method 21
(3) MIC (minimum inhibitory concentration) 22
(4) MBC (minimum bactericidal concentration) 22
5. 통계 처리 22
III. 결과 및 고찰 23
1. 분리된 화합물의 구조 분석 23
1) Compound 1 23
2) Compound 2 26
3) Compounds 3-5 29
2. 생달나무 잎 추출물 및 분획물의 활성 실험 결과 35
1) 항산화 35
(1) 총 폴리페놀 함량 35
(2) 총 플라보노이드 함량 36
(3) DPPH radical 소거 활성 37
(4) ABTS+ radical 소거 활성 38
2) 항염 39
(1) Nitric oxide (NO) 생성 억제 활성 39
(2) Western blot analysis 42
(3) 전염증성 cytokines (IL-1β, TNF-α) 생성 억제 활성 44
3) 항균 46
(1) Paper disc diffusion method 46
(2) MIC 및 MBC 47
3. 분리된 화합물의 활성 실험 결과 49
1) 항염 49
(1) Nitric oxide (NO) 생성 억제 활성 49
(2) Western blot analysis 52
(3) 전염증성 cytokines (IL-6, IL-1β, TNF-α) 생성 억제 활성 53
Ⅳ. 요약 및 결론 55
Ⅴ. 참고문헌 57
Degree
Master
Publisher
제주대학교 대학원
Citation
김소희. (2020). 생달나무 잎 추출물의 항산화, 항염 및 항균 활성 성분 연구
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