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Pentadecanoic acid as a novel anti-cancer agent in human breast cancer stem-like MCF-7/SC cells

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Author(s)
Ngoc Bao To
Issued Date
2021
URI
https://oak.jejunu.ac.kr/handle/2020.oak/23499
Abstract
Compelling evidence from biological and nutritional studies recently has further reinforced the importance of odd-chain fatty acids (OCFAs) in health benefits. Very little is known about the biological mechanism of OCFAs as anti-cancer agents. Pentadecanoic acid (C15:0) is one of the common OCFAs. In this study, we found that pentadecanoic acid could suppress the stemness of the breast cancer stem-like cells MCF-7/SC. MCF-7/SC were established from MCF-7 breast carcinoma cells, which exhibit high stem cell properties. Our results demonstrated that pentadecanoic acid has specific cytotoxicity on MCF-7/SC compare to its parental cells. In addition, pentadecanoic acid dramatically suppressed the stemness by targeting CSC markers, mammosphere formation, and migration as well as invasion ability of MCF-7/SC cells. Moreover, we found that pentadecanoic acid can suppress IL-6-induced JAK2/STAT3 activation, induce sub-G1 accumulation and promote caspase-dependent apoptosis in breast MCF-7/SC cells. In addition, pentadecanoic acid enhanced the chemosensitivity of MCF-7/SC cells to tamoxifen, a commonly used chemotherapy in breast cancer treatments. These findings indicate that pentadecanoic acid can serve as a novel JAK2/STAT3 signaling inhibitor in breast cancer cells and suggest the beneficial effects of pentadecanoic acid-rich food intake during breast cancer treatments.
Affiliation
제주대학교 대학원
Department
대학원 차세대융복합과학기술협동과정
Advisor
김소미
Awarded Date
2021. 2
Table Of Contents
ABSTRACT . 1
1. INTRODUCTION . 2
2. MATERIALS AND METHODS 5
2.1. Cell line and cell culture . 5
2.2. Cell viability assay 5
2.3. Cell migration assay . 5
2.4. Cell invasion assay 6
2.5. Flow cytometric analysis of the CD44+/CD24 population 6
2.6. ALDEFLUOR assay 7
2.7. Mammosphere formation assay 7
2.8. Cell cycle assay 7
2.9. Annexin V/PI staining assay 8
2.10. Western blot analysis 8
2.11. ROS generation analysis 8
2.12. Statistical analysis . 9
3. RESULTS 10
3.1. MCF-7/SC cells exhibited higher stem cell properties than parental MCF-7 cells . 10
3.2. Pentadecanoic acid effectively inhibits MCF-7 & MCF-7/SC cell proliferation. 12
3.3. Pentadecanoic acid supressed migration and invasion capacity of MCF-7/SC cells . 15
3.4. Pentadecanoic acid suppressed the stem cell-like properties of MCF-7/SC cells 17
3.5. Pentadecanoic acid suppress cancer the JAK2/STAT3 signaling in MCF-7/SC cells. 19
3.6. Pentadecanoic acid induce apoptosis in MCF-7/SC cells 22
3.7. Pentadecanoic acid enhances chemosensitivity of MCF-7/SC cells to tamoxifen 24
4. DISCUSSION 28
5. CONCLUSIONS . 34
REFERENCES . 35
Degree
Master
Publisher
제주대학교 대학원
Citation
Ngoc Bao To. (2021). Pentadecanoic acid as a novel anti-cancer agent in human breast cancer stem-like MCF-7/SC cells
Type
Dissertation
Appears in Collections:
General Graduate School > Interdisciplinary Graduate Program in Advanced Convergence Technology and Science
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