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Analysis of changes in carotenoid biosynthesis in micro-tom tomatoes introduced with citrus CCD1A gene.

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Author(s)
Jeong, Dong Sik
Issued Date
2021
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000010409
Abstract
During the ripening process of citrus fruits, the orange color is caused by the accumulation of carotenoid pigments. Through differentially expressed gene(s) screening, the carotenoid cleavage dioxygenase (CCD) gene was isolated as a pigment-related gene with higher expression than the control group through gene analysis in the citrus eggplant mutant red fruit gene and in the citrus control group. CCD, which decomposes carotenoid metabolites, is an enzyme that catalyzes the reaction of bonding two oxygen atoms to a substrate by cleaving the double bond of C40 carotenoid. Four types have been reported in plants so far. CCD1 and CCD4 have broad substrate specificities ranging from phytoene to neoxanthin, CCD7 and CCD8 are involved in the synthesis of stringolactone from β-carotene, and 9-cis-epoxycarotenoid dioxygenases NCED2, NCED3, NCED5, NCED6, and NCED9 produce abscisic acid.
In this study, the expression changes of carotenoid biosynthesis genes were analyzed in transgenic Micro-Tom tomatoes isolated from citrus fruits introduced with CCD1A gene, total carotenoid content, flavonoid content, and phenolic compound content were analyzed, and antioxidant activity was analyzed to analyze CCDA1 The influence by genes was investigate.
Affiliation
제주대학교 대학원
Department
대학원 바이오소재공학과
Advisor
김인중
Awarded Date
2021. 8
Table Of Contents
1. Introduction 1
2. Materials and Methods 5
2-1. Plant materials
2-2. Genomic DNA extraction from seed.
2-3. Genomic DNA extraction from leaves.
2-4. Total RNA extraction and DNase treatment
2-4-1. RNA extraction
2-4-2. DNase treatment
2-5. PCR (Polymerase Chain Reaction) & Electrophoresis
2-5-1. Genotyping of T3 S-CuCCDA1 / T3 AS-CCDA1 lines from single seed
2-5-2. Genotyping of T3-S-CuCCDA1 / T3-AS-CuCCDA1 lines from leaf
2-5-3. cDNA synthesis
2-6. Anti-oxidant activity analysis
2-6-1. Total carotenoid content
2-6-2. Total Flavonoid content
2-6-3. Total Phenolic Content
2-6-4. DPPH radical scavenging
2-7. HPLC(High Performance Liquid Chromatography)
3. Result 19
3-1. Genotyping analysis of T3-S/AS-CuCCDA1 transformant lines using genomic DNA from single seed
3-2. Genotyping analysis of T3-S/AS-CuCCDA1 transformant lines using genomic DNA from single seed
3-3. Total RNA extraction and DNase treatment
3-4. RT-PCR
3-5. Anti-oxidant activity analysis
3-5-1. Total carotenoid content
3-5-1. Total Flavonoid content
3-5-2. Total Phenolic Content
3-5-3. DPPH radical scavenging
3-6. HPLC(High Performance Liquid Chromatography)
4. Summary and Conclusions 60
Degree
Master
Publisher
제주대학교 대학원
Citation
Jeong, Dong Sik. (2021). Analysis of changes in carotenoid biosynthesis in micro-tom tomatoes introduced with citrus CCD1A gene.
Type
Dissertation
Appears in Collections:
General Graduate School > Biomaterials Science and Technology
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