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Effect of Methanolic Extracts of Acer Cappadocicum on HepG2 Cancer Cell Line in a Liver Microphysiological System

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Abstract
The liver is a vital organ for many crucial functions within the human body, and should it become diseased or injured, the loss of those integral functions can cause real damage to the human cells. Therefore, hepatotoxicity measurement of any plant extract is essential because liver toxicity such as hepatitis, cirrhosis, and hepatocellular carcinoma affect human life and health because they alter the metabolism and detoxification of the human body. Thus, this study depicted the estimation of the hepatotoxicity potentials of an accessed Acer cappadocicum (AC) population plucked from the Kohistan Valley mountains in northern Pakistan. The leaf extracts of the plant were made in organic solvents of different polarities. The other proportion ratios of extracts were prepared through the process of maceration. Hepatotoxicity is griping about evaluating for any anticancer drug toxicity; this study was directed by applying a liver chip to estimate the hepatotoxicity assessment. We first assembled a bilayer liver MPS by executing the four hepatic cell line types (HepG2 cancer cell line, HUVEC, stellate cells, and Kupffer cells) in a bilayer microchannel with the help of laminal flow in the microchannel using micropumps. This chip was fabricated and operated by MPS protocol. It also described the better hepatic activity (ROS synthesis, and liver enzyme activities. Then, we added Methanolic extracts of A. cappadocicum into the chip. It successfully accessed the hepatotoxic effect of this A. cappadocicum in the organ-on-a-chip. Methanolic extracts of Acer cappadocicum were administrated to anticancer and antioxidant activities. Still, they accessed its hepatotoxicity using the HepG2 cancer cell line in the Liver Micro-physiological system, impacting ROS, and SOD, ALT, and AST assays. A. cappadocicum extract vigorously inhibited ALT and AST levels. Thus, this extract decreased the growth of enzymes in hepatocytes. The inhibitory evidence of extracts showed the ability and low level of enzymes viability of the HepG2 cancer cell line.
Author(s)
Farooqi, Muhammad Awais
Issued Date
2022
Awarded Date
2022. 2
Type
Dissertation
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000010498
Alternative Author(s)
파루키 무하마드 아와이즈
Affiliation
제주대학교 대학원
Department
대학원 에너지응용시스템학부 메카트로닉스공학전공
Advisor
Kang, Chul Ung
Table Of Contents
Abstract 6
Introduction 7
1. MPS platforms of the liver HepG2 Cancer Cell Line 10
1.1 Opportunities for integrating MPS platforms 12
1.2. Perspectives on Integration 14
2. Liver-On-Chip (LOC) Platforms 16
2.1. Liver-On-Chip Platforms Based on Immortalized cell lines 18
2.2. Microphysiological systems (MPSs) are microengineered cell culture platforms 19
2.3. Liver MPS used in the current study 21
3. Liver MPS used in the current study 22
4. Materials and Methods 23
4.1. Cell seeding and liver fibrosis on microfluidic chip 25
4.2. ECM evaluation in the liver-on-chip device 26
4.3. ROS, SOD, AST, and ALT enzyme measurements 27
4.4. Fabrication of microfluidic device 28
4.5. Cell culture and seeding on the microfluidic chip 29
5. Results 42
6. Discussion 46
7. Conclusion 50
Degree
Master
Publisher
제주대학교 대학원
Appears in Collections:
Faculty of Applied Energy System > Mechatronics Engineering
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