마우스 세포에서 Acenocoumarol의 멜라닌 생성 억제 및 항염증 효과 규명

Abstract

In this study, coumarin derivatives acenocoumarol and warfarin demonstrated the inhibition and anti−inflammatory effects of melanogenesis in mouse cells. The effect of inhibiting melanogenesis was confirmed in B16F10 cells, and both acenocoumarol and warfarin significantly reduced melanin production. Subsequently, the effect of acenocoumarol on the signal transmission path in B16F10 cells was investigated. Acenocoumarol inhibited the expression of melanin−producing enzymes (tyrosinase, TRP−1, TRP−2). And it was confirmed that the expression of MITF, which regulates the production of tyrosinase, TRP−1, and TRP−2, was suppressed. In addition, it was confirmed that the expression of MITF was suppressed through various signal transmission paths, and finally melanin production was suppressed. Therefore, inhibiting the production of excessive melanin through the regulation of various signaling pathways helps prevent various diseases and can be considered a promising strategy for the treatment of hyperpigmentation and the development of whitening agents. In addition, anti−inflammatory effects of acenocoumarol were investigated in LPS−stimulated RAW 264.7 cells, which significantly reduced the expression of iNOS and COX−2 through various signaling pathways and ultimately reduced the production of inflammatory agents (NO, PGE2) and pro−inflammatory cytokines. Therefore, inhibiting the activation of macrophages and the generation of inflammatory mediators can be seen as a promising strategy for improving inflammatory diseases and developing anti−inflammatory treatments.