마우스 B16F10 세포에서 쿠마린 유도체 Isoimperatorin과 Imperatorin의 멜라닌 생성 증진 효과

Abstract

This study investigated the melanogenesis effect of isoimperatorin and imperatorin and the basic signaling pathway of imperatorin. The coumarin derivatives isoimperatorin and imperatorin were tested at concentrations of 6.25, 12.5 and 25 μM, which do not affect cell viability. Isoimperatorin and imperatorin increase melanin in the evaluation of melanin content and tyrosinase activity. Interestingly, there was no difference in melanin content and tyrosinase activity, despite the structural differences between isoimperatorin and imperatorin. Imperatorin increased melanin by regulating Tyrosinase, TRP-1 and TRP-2 enzymes, which play an important role in melanin activity. MITF is a protein factor that plays a major role in melenogenesis. Imperatorin increases melanin synthesis by finally regulating MITF through the ERK pathway of MAPKs, PKA/CREB pathway and AKT/GSK3β/β-catenin pathway in B16F10 cells. These pathways were further identified using specific inhibitors. In addition, it was confirmed that melanin production was induced in human epidermal melanocyte cells by treating imperetorin in HEMn-MP cells. Finally, the primary stimulation evaluation of the human skin was conducted to confirm whether the use of imperatorin had a stimulation or sensitization reaction to the skin. Through this test, it was confirmed that imperatorin was a hypoallergenic substance. In conclusion, the activity of coumarin derivatives on melanin production was evaluated through research. Both isoimperatorin and imperatorin showed increased melanin, and especially imperatorin can be used as a local treatment to treat hypochromic deposition.