제주도 사육 한우에서 타일레리아와 아나플라즈마에 관한 분자역학조사

Abstract

This study investigated Theileria and Anaplasma spp. infections of Korean native cattle in Jeju Island using molecular diagnostic tests. Blood samples were collected from 148 Korean native cows in six areas of Jeju Island and the infection rate of each pathogen was confirmed via polymerase chain reaction (PCR) using pathogen-specific primer sets. In June 2022, the infection rate of T. orientalis and Anaplasma spp. for 100 cows in Hawon-dong, Seogwipo-si, was 96% (96/100) and 91% (91/100), respectively. Co-infection rate of the two pathogens was 79.7%. According to region, the infection rate of T. orientalis was high in Hallim-eup, Andeok-myeon (100%), Hawon-dong (96%), and Pyoseon-myeon (90%), whereas that of Nohyeong-dong was the lowest at 30%. In Anaplasma spp., the infection rate was high in Pyoseon-myeon (100%), Hawon-dong (91%), and Andeok-myeon (80%), and that of Nohyeong-dong was the lowest at 10%. It is difficult to determine the differences in rates by region owing to differences in the distribution of individuals in each region, but infection rates increased as age increased. Sequencing analysis showed that the homogeneity of each PCR product was 98.43% and 98.03% with T. orientalis (Genbank accession no. U48772.1) and Anaplasma phagocytophilum (Genbank accession no. NR.04762.1), respectively. A preventive policy for vector-borne diseases caused by Theileria and Anaplasma spp. is required because the productivity of livestock could be affected, resulting in economic loss. Because Anaplasma phagocytophilum is zoonotic, continuous monitoring for the pathogen is required.