병풀추출물을 함유한 지질 기반 나노베지클의 특성과 피부투과 연구

Abstract

In this study, the physicochemical characteristics and skin permeability of various forms of liposomes containing centella asiatica extract were compared. The physicochemical properties and skin absorption rates of liposome and tranfersome using various surfactants as edge activators, ethosome prepared by varying the content of ethanol, and transethosome containing various edge activators and ethanol were compared. The particle size, PDI, zeta potential, and pH of the nano vesicles were observed at 25℃ and 45℃ for 60 d. First, after 60 d of manufacture, the particle size of liposome and tranfersome did not change significantly at 25℃, but the size of the transfersome containing span 80 (HLB 4.3) and Tween 85 (HLB 11) increased, and the smaller the surfactant HLB value, the particle size tended to increase. This is believed to be due to the Ostwald ripening in which when the HLB value of the non-ionic surfactant is large, the surfactant in the small particles melts into the medium more and is adsorbed to the large particles. It was confirmed that the zeta potential, PDI, and pH were stable because there was no significant change from 25℃. Ethosome confirmed that the higher the content of ethanol, the higher the particle size and PDI. It was confirmed that the zeta potential decreased slightly when stored at a 45℃, and the pH did not change significantly. Through this, when the content of ethanol is 5%, the particle size is the smallest and the PDI and zeta potential are stable. Through the optimization of ethosome, it was confirmed that the content of ethanol was the most stable when the ethanol was 5%, and transethosome was referred to in manufacturing. Span 80, Tween 20, Tween 80, and Tween 85 were used as edge activators, and as a result, it was confirmed that the particle size was changed by the edge activator. The particle size tends to increase as the surfactant HLB value decreases, and the particle size is the smallest particle size containing Tween 20 with the highest HLB value. It was confirmed that PDI, zeta potential, and pH were stable without significant change. Through this, the optimized conditions were confirmed, and the ratio of phospholipids to surfactants was 80:20, and when Tween 20 was used, it was judged to be the most stable formulation when the ethanol content was 5%. These results were prepared by liposome, transfersome, ethosome, and transethosome that perform the capsule efficiency, drug release, skin absorption, and TEM. In the capsule efficiency experiment, the efficiency was high in the order of ethosome, transethosome, liposome, and transfersome, and in the drug release experiment, tranfersome was the highest at 46.39±0.16% after 2 h, and the most release occurred at 94.90±0.26% after 24 h. After 24 hours, liposome, transehtosome, and ethosome appeared in the order, it was confirmed that the drug was slowly released from four formulations, and it was judged to be an appropriate formulation to deliver the drug. As a result of evaluating the skin permeability of the four formulations using Franz diffusion cell, the skin permeability of transethosome was the highest, followed by transosome, ethosome, and liposome. At the skin permeation rate (Flux), the speed of liposome and ethosome increased after 2 h of initial permeation, but after 4 h of permeation, it was confirmed that the permeation speed of transethosome and transosome increased rapidly. This reduces the interfacial tension of the lipid membrane present in the stratum corneum because transethosome has both variability and flexibility by surfactants and ethanol, and as the skin barrier loosens, the delivery of active substances into the skin is effectively delivered deeper. Cryo-TEM was used for morphological observation of the four formulations, all of which identified lipid double layers, and the vesicle size of 90-150 nm. In addition, when the skin absorption rate was investigated according to the type of phospholipid, the skin absorption rate was higher when using non-hydrogenated Lipoid S75 and Lipoid P100 than the hydrogenated Lipoid S75-3, and the overall absorption rate of transethosome was the highest. However, it was found that raw materials that were not hydrogenated were difficult to store, so it was not easy to use. Through this study, the physicochemical and morphological characteristics of the four formulations were investigated, and through skin permeation experiments, transethosome with both variability and flexibility had the highest skin permeability and flux. Experiments on various formulations have confirmed that nano vesicles containing bottled grass quantitative extracts can be used as a cosmetic formulation, and it can be expected to play a role not only in cosmetics but also in locally affecting skin problems.