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당근 세균잎마름병에 대한 효과적 방제 수단

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Abstract
Bacterial blight of carrot caused by Xanthomonas hortorum pv. carotae (Xhc) is one of the serious diseases of carrot, of which control measures has not been still established in the domestic farm. In this study, in order to select effective sterilizer for bacterial blight of carrots, three antibiotics such as streptomycin, oxolinic acid, kasugamycin, two copper compounds like copper hydroxide and copper sulfate basic and three rhizobacteria Burkholderia gladioli MRL408-3, Pseudomonas fluorescens TRH415-2 and Bacillus cereus KRY505-3 were selected to investigate their direct antibacterial effects using artificial media, aiming to identify effective pesticides against Xhc. Among them, treated medium with antibiotics such as streptomycin, oxolinic acid, and the antagonistic rhizobacteria MRL408-3 were formed inhibition zone. The agrochemicals and the rhizobacteria MRL408-3, which showed antibacterial effects on carrot leaves, pre-treated on the carrot leaves and then inoculated with Xhc. High control effects were shown on the carrot leaves pre-treated with both streptomycin and oxolinic acid. Scanning electron microscopy images of the carrot leaf surfaces showed that the population of bacteria decreased significantly on leaves pre-treated with streptomycin and oxolinic acid. To control bacterial leaf blight, a seed-borne disease the agrochemicals which showed antibacterial effects on carrot leaves, pre-treated on the carrot seeds, and then inoculated with Xhc. Among them, high control effects were shown on the carrot seeds pre-treated with in order oxolinic acid and streptomycin. From these results, it can be inferred that antibiotics like streptomycin and oxolinic acid exhibit superior control effects compared to other agents and antibiotics like streptomycin and oxolinic acid exhibit superior control effects on carrot seeds compared to other agents. This study provides valuable insights towards establishing an effective control system for bacterial blight of carrot.
Author(s)
강현수
Issued Date
2024
Awarded Date
2024-02
Type
Dissertation
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000011630
Alternative Author(s)
Kang HyunSu
Affiliation
제주대학교 대학원
Department
대학원 농학과
Advisor
전용철
Table Of Contents
Ⅰ. 서 론 2
Ⅱ. 재료 및 방법 6
ⅰ. 당근 잎에서 방제 효과 실험
1. 당근 식물체 및 재배조건
2. 식물병원성 세균 및 배양조건
3. 당근 세균잎마름병균에 대한 항균활성 실험
4. 당근 세균잎마름병 방제 효과 평가
5. 장방출주사현미경을 이용한 당근 세균잎마름병균을 접종한 당근 잎 관찰
ⅱ. 종자에 병원균 접종 및 방제 효과 실험
1. 당근 종자에 당근 세균잎마름병균 접종 시험
2. 당근 종자에 접종한 균 당근 잎에서 재분리
3. 분리세균 DNA 추출
4. PCR 증폭
5. 당근 종자에 방제 효과 평가
ⅲ. 통계분석
Ⅲ. 결 과 18
ⅰ. 당근 세균잎마름병 방제 효과 검정
1. 시판 농약과 식물근권세균의 당근 세균잎마름병균에 대한 항균활성
2. 생체 실험을 통한 시판 농약과 식물근권세균의 당근 세균잎마름병에 대한 방제 효과
3. 장방출주사현미경을 이용한 시판 농약에 의한 당근 세균잎마름병 방제 효과 검정
ⅱ. 종자에서 방제 효과 검정
1. 당근 종자 접종을 통한 발병도 및 세균 분포
2. 분리 세균의 분자생물학적 동정
3. 종자를 통한 시판 농약의 당근 세균잎마름병에 대한 방제 효과
Ⅳ. 고 찰 37
Ⅴ. 적 요 41
Ⅵ. 참 고 문 헌 43
Degree
Master
Publisher
제주대학교 대학원
Citation
강현수. (2024). 당근 세균잎마름병에 대한 효과적 방제 수단.
Appears in Collections:
General Graduate School > Agricultural Science
공개 및 라이선스
  • 공개 구분공개
  • 엠바고2024-02-12
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