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Oleuropein Rich Olive Leaf Curtails PM-Induced Allergic Airway Inflammation in Mouse

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Abstract
PM is well-known as an asthma aggravating crucial factor frequently existing in air pollutants. Oleuropein rich olive leaf (Olea europea) extract (OLE) has been recog- nized as an effective therapeutic compound to suppress multiple health issues includ- ing inflammatory conditions in respiratory tract. This study was mainly focused on exploring beneficial suppressive abilities of olive leaf for asthma inflammations. Dur- ing this study, we developed a mouse model, by intraperitoneally injecting BALB/c mice with Ovalbumin (OVA) followed by challenge with PM (30mins/day) for 7 days consecutively in a nebulizer. Afterwards, the oral gavage of OLE and the positive con- trol, prednisone was done. As results, we observed that the OLE significantly de- creased eosinophil and basophil infiltration in blood and BALF, further inflammatory cell infiltration and more specifically eosinophil infiltration in both lung and trachea which were aberrantly increased by PM. OLE abated PM-induced TLR4 expression. This proceeds to reduced mRNA expression of pro-inflammatory cytokines by Th1 response (IL-1β, TNF-α and IFN-γ) and inflammatory cytokines by Th2 and Th17 re- sponse (IL-4, IL-10, IL-13, IL-17, and IL-22) resulting reduced mucus secretion in airway epithelia and reduced inflammatory conditions. In conclusion, the Olive leaf extract is proficient in alleviating inflammatory conditions by suppressing Th2 and Th17 cell activation, mast cell degranulation and mucus secretion in PM-induced al- lergic asthma by rectification of TLR4 induced signalling as a substituting conven- tional drug. Key words: Particulate matter, Olive leaf extract, Oleuropein, allergic asthma, Th2 and Th17 response
Author(s)
아트나야크 락세라
Issued Date
2024
Awarded Date
2024-02
Type
Dissertation
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000011864
Alternative Author(s)
Aththanayaka Laksara Punnya Keshari
Affiliation
제주대학교 대학원
Department
대학원 차세대융복합과학기술협동과정
Advisor
Jee Young heun
Table Of Contents
Abstract 1
Introduction 2
Research Hypothesis 7
Aim 8
Materials and methods 9
Mice 9
Particulate matter 9
Plant extract of Olive leaf 11
Animal model . 12
Contrast of Differential cell quantity of blood in asthma induced mice exposed to PM 13
Contrast of Differential cell quantity of Bronchoalveolar Lavage Fluid (BALF) in asthma induced mice exposed to PM 13
Histopathological analysis of inflammatory cell infiltration in trachea and lung in asthma induced mice exposed to PM 14
Immunohistochemistry analysis of lung and trachea in asthma induced mice exposed to PM 14
RNA extraction, cDNA synthesis and real-time PCR analysis in asthma induced mice exposed to PM 15
Results 17
OLE diminishes Differential cell quantity of BALF in asthma induced mice exposed to PM 19
OLE curtails histopathological inflammatory cell infiltration in asthma induced mice exposed to PM 21
OLE drastically reduces eosinophil infiltration in asthma induced mice exposed to PM 23
OLE attenuates mast cell degranulation in asthma induced mice exposed to PM 25
OLE involves in masking the expression of Toll-Like Receptors (TLRs) in asthma induced mice exposed to PM . 27
OLE mitigates anti-inflammatory cytokine production from Th2, Th17 cells in asthma induced mice exposed to PM. 29
OLE suppress the mucus hypersecretion from goblet cells in asthma induced mice exposed to PM 31
OLE downgrade MUC5AC over expression lung in asthma induced miceexposed to PM 33
Discussion 36
Conclusion 40
References 41
Degree
Master
Publisher
제주대학교 대학원
Citation
아트나야크 락세라. (2024). Oleuropein Rich Olive Leaf Curtails PM-Induced Allergic Airway Inflammation in Mouse.
Appears in Collections:
Interdisciplinary Programs > Interdisciplinary Graduate Program in Advanced Convergence Technology and Science
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