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Effect of feeding very low carbohydrate (horsemeat- or beef- based) diets and re-stricted feeding on weight gain, feed and energy efficiency, and serum levels of cholesterol, triacylglycerol, glucose, insulin and ketone bodies in adult rats

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Author(s)
김재연
Issued Date
2007
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000004106
Abstract
Studies were carried out to determine the effect of very low carhohydrate diets and restricted feeding (70% ad lib intake) on weight gain, feed efficiency and serum levels of cholesterol, triacylglycerol, glucose, ketone bodies and insulin. Forty rats (mean initial weight 238g) or 30 rats (251g) were divided into groups of 10 rats each and fed for four weeks or 26 days in exp. 1 or 2, respectively. In exp. 1 two groups were assigned to a conventional diet (AIN-93G) or a very low carbohydrate (LC) diet containing 10% starch and 53% lyophilized ground horsemeat, and one of the two groups was fed ad lib and the other pair-fed 70% on the basis of metabolic body size of the ad lib intake. In exp. 2 each of the three groups was assigned to a control (AIN-93G) or diets containing 10% starch and 53% beef or horsemeat. Average daily body weight gain was not different between the two diets in exp. 1, whereas in exp. 2 was lower in rats fed the AIN-93G than in those fed the very low carbohydrate diets at beginning, but was reversed toward the end of the feeding period. Rats with restricted intake gained much less weight (P<0.01) than those had ad lib intake in exp. 1. Feed intake was higher in rats fed AIN-93G diet than those fed LC diet (P<0.01) in both experiments, whereas energy intake was not different between the two diets in exp. 1. LC diet reduced (P<0.01) serum triacylglycerol compared with AIN-93G diet and restricted feeding also reduced serum triacylglycerol, glucose and insulin levels especially in rats fed AIN-93G diet (P<0.05 for diet × feeding level) compared with ad lib intake in exp. 1. Serum LDL cholesterol level was increased by restricted feeding (exp. 1) or by LC diet (exp. 2), compared with ad lib intake or AIN-93G, respectively. Body composition was not affected by diet but protein content was increased with a concomitant decrease in fat content by restricted feeding. Serum ketone body level was higher in rats fed LC diet than in those fed AIN-93G diet in exp. 2. No differences in all the parameters determined were found between the horsemeat- and beef-based diets in exp. 2. Results indicate that feeding LC diet is beneficial for alleviating risk factors, such as serum triacylglycerol level known to involve cardiovascular diseases or artherosclerosis and restricted feeding with still gaining weight is highly effective in reducing serum triacylglycerol level. However, more studies with model animals as well as humans are required to examine long-term health benefits of low carbohydrate diets.
이 연구는 체중증가, 사료효율, 혈청 내 콜레스테롤, 중성지방, 글루코스, 케톤체, 인슐린에 미치는 저탄수화물 사료와 제한급여 (무제한 급여량의 70%)의 영향을 측정하기 위해 수행되었다. 실험1과 실험2에서 실험쥐 (Sprague-Dawley rat) 각 40마리 (평균체중 238 g)와 30마리 (평균체중 251g)를 10마리씩 나누고 각각 4주와 26일 동안 사료를 급여하였다. 실험 1에서, 두 그룹의 쥐에게는 보통사료(AIN-93G), 다른 두 그룹의 쥐에게는 10% 전분과 53% 냉동 건조된 분말 말고기가 포함된 저탄수화물 사료 (LC)를 급여하였다. 두 그룹 중 하나를 무제한 급여하였고 다른 하나를 무제한 급여 그룹과 짝을 지어 무제한 급여 그룹의 섭취량과 대사체중에 기초하여 70%에 해당하는 양을 급여하였다. 실험 2에서는 세 그룹에 각각 대조구 (AIN-93G) 또는 10% 전분과 53% 냉동 건조된 분말 말고기 또는 쇠고기가 함유된 사료를 급여하였다. 실험 2에서 평균 일일 체중 증가가 급여기간 초기에 저탄수화물 사료가 급여된 쥐에서 보다 대조구 사료가 급여된 쥐에서 낮은 반면 실험 1에서는 두 사료 사이에 차이가 없었다. 실험 1에서 제한 급여 그룹이 무제한 급여 그룹보다 체중감소가 더 컸다. 사료 섭취량은 두 실험 모두 저탄수화물 그룹보다 대조구 diet 그룹에서 높았지만 (P<0.01) 실험 1에서 에너지 섭취량은 두 diet 간에 차이가 없었다. 실험 1에서 저탄수화물그룹이 대조구 그룹보다 혈청 중성지방이 감소하였고 (P<0.01) 대조구 그룹에서 무제한 급여 그룹보다 제한 급여 그룹에서 혈청 중성지방, 글루코스, 인슐린 수준이 현저하게 감소하였다 (P<0.05). 혈청 LDL 콜레스테롤 수준은 제한급여 (실험1) 또는 저탄수화물 그룹(실험2)에서 증가하였다. 체조성은 diet간에 차이는 없었지만 제한급여에서 지방 양은 감소하면서 단백질 양은 증가하였다. 혈청 케톤체 수준은 실험 2에서 AIN-93G diet 그룹보다 LC diet 그룹에서 높았다. 실험 2에서 모든 측정치에서 소고기와 말고기 그룹간 차이가 없었다. 결과적으로 LC diet 섭취는 심혈관 질환이나 동맥경화에 관계되는 혈청 중성지방 수준 같은 질병요인을 완화시키는데 유익하고, 제한급여는 혈청 중성지방 수준을 현저하게 감소시킨다는 것을 알아내었다. 그러나 앞으로 인간과 모델 동물을 사용하여 저탄수화물 식이의 장기간 섭취가 건강에 어떤 영향을 미치는지 연구를 계속할 필요가 있다.
Affiliation
제주대학교
Department
대학원 동물자원과학과
Advisor
김규일
Awarded Date
2007. 8
Table Of Contents
Ⅰ. INTRODUCTION 3
Ⅱ. LITERATURE REVIEW 5
Very low carbohydrate diet 5
Restricted feeding 7
Ⅲ. MATERIALS AND METHODS 9
Experiment 1. 9
1. Animals and diets 9
2. Analysis of cholesterol, triacylglycerol, glucose, and insulin in serum 11
3. Proximate analysis of feed and carcass samples 11
4. Statistical analysis 12
Experiment 2. 13
1. Animals and diets 13
2. Analysis of cholesterol, triacylglycerol, glucose, ketone bodies and insulin in serum 13
3. statistical analysis 14
Ⅳ. RESULTS 15
Experiment 1. 15
Experiment 2. 21
Ⅴ. DISCUSSION 25
Ⅵ. ABSTRACT 28
REFERENCES 30
Degree
Master
Publisher
제주대학교
Citation
김재연. (2007). Effect of feeding very low carbohydrate (horsemeat- or beef- based) diets and re-stricted feeding on weight gain, feed and energy efficiency, and serum levels of cholesterol, triacylglycerol, glucose, insulin and ketone bodies in adult rats
Type
Dissertation
Appears in Collections:
Faculty of Biotechnology > ETC
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