관절액이 골수 기원 줄기세포 생존 및 증식에 미치는 영향 분석

Abstract

Purpose : The aim of this study is to inspect The effect of human joint-fluid media on the survival and proliferation of bone-marrow derived progenitor cell and to provide the basic data of the intra-articular injection with scaffold-free progenitor cell in advanced degenerative osteoarthritis(OA).

Materials and Methods : We obtained the joint fluid and bone marrow from 15 patients who had total knee arthroplasty due to degenerative OA, and isolated the mesenchymal progenitor cells(MPCs) from bone marrow by washing and ten times subculture. We devided the control and experiment groups according to the addition of joint fluid at various ratios(1/100, 1/10, 1, 10, 100, 1000㎕), and statistically analyzed the numbers of mesenchymal progenitor cell proliferated according to the culture period.

Results : The experiments using joint fluid without centrifuge showed the increase of MPCs as the culture period was extended and was independent to the existence of fetal bovine serum, the dose dependent pattern in the increase of MPCs in proportion to the of joint fluid, and statistically significant increase MPCs in 10, 100,1000㎕ of serum contained groups, 1000㎕ of serum-free groups (p=0.039, p=0.017, p=0.077, p=0.004). The experiments using joint fluid with centrifuge showed the increase of MPCs as the culture period was extended and was independent of fetal bovine serum, and the dose dependent pattern in the increase of MPCs in proportion to the dose of joint fluid, and statistically significant increase MPCs in 1000㎕ of both serum contained and serum-free groups (p=0.006, p=0.024).

Conclusion : MSCs not only can survive, but also proliferate in human joint fluid. The rate of proliferation is increased faster by the adding of joint fluid than only using common media in cell culture. And the experiment shows the dose dependent pattern in the increase of MPCs in proportion to the dose of joint fluid.