한우 체외 수정란 발육 및 초자화 동결시 α-Tocopherol 첨가효과에 대한 硏究

Abstract

This study was undertaken to find if supplementation of vitamin E in culture medium could ameliorate the developmental competence of preimplantation Hanwoo embryos. Methods α-tocopherol was supplemented in maturation / embryo culture medium at concentrations of 0, 100, 200 and 400 µM. The developmental competence of Hanwoo embryos were assessed by observing the cleavage, blastocyst rate, total cell number and cell count after vitrification. Cleavage rate from July to October compared to the results, α-tocopherol 200 µM supplemented with α-tocopherol 200 µM treatment groups and was higher than the other treatments that had no significant effect in maturation medium. In the blastocyst rate of August to October, α-tocopherol in embryo culture medium. Treatment groups containing α-tocopherol 200 µM 38.60±7.12% were significantly higher(p<0.05) than control and other treatments. Before and After vitrification of the total cell numbers in treated α-tocopherol 200 μM, 115.80±6.61, 106.33±3.50, respectively, and control cells was higher than the other treatment groups. According to monthly, the blastocyst rate and the freezing of embryos in the total cell number that July to August was significantly higher(p<0.05) than September to October. These results suggest that the addition of antioxidants in vitro bovine embryo production and cryopreservation may be beneficial in the future to identify and freeze the other way as antioxidants to improve the quality looks to be able to produce embryos.