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항산화성 천연화합물이 neuraminidase 및 superoxide dismutase외 catalase의 활성에 미치는 영향

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Abstract
Effects of flavonoid compounds and 70% ethanol extract of a parsley (Petroselinum crispum), known have rich with apigenin, on viral and bacterial neuraminidase activity were investigated.
Most of polyphenolic and flavonoid compounds from a parsley were extracted within 1 hour of extraction with 70% ethanol.
Flavonoid compounds inhibited bacterial neuraminidase, but interpretation of the data needs caution due to the experimental standard errors. Among those apigenin and zanamivir showed relatively higher enzyme inhibition ratio.
Apigenin showed higher inhibition activity against viral neuraminidase than zanamivir, a flu medicine. Ethanol extract of a parsley also inhibited viral neuraminidase as zanamivir did.
Apigenin was suggested to exist as apiin, a glycoside form, in parsley. Its ethanol extract was hydrolyzed by acid, and the hydrolysate was analyzed by high pressure liquid chromatography(HPLC). The ethanol extract was found to contain apigenin 55 ㎍/㎎ based on HPLC chromatogram. Mass spectra of the acid hydrolysate showed peaks at 270.9 and 272.1 m/z corresponding to apigenin.
High pressure-solvent extracts of such plants as Rhus chinensis, Malus sieboldii and Ostrya japonica, containing antioxidative poplyphenol compounds were examined for their in vivo effects on superoxide dismutase(SOD) and catalase(CAT) level using 6-week old Sprague-Dawley(SD) rat.
Body and organ weights of rats, fed with AAPH[2,2′-azobis(2-methylpropionamidine) dihydrochloride] alone or with AAPH and plant extracts, decreased being compared with those of rats fed with normal diet.
SOD levels in blood serum and liver increased when AAPH was administered, and these increases were not reversed by feeding any of the plant extracts. On the contrary, plant extracts showed tendency of increasing SOD level.
CAT levels in blood serum and liver were higher in rats fed with AAPH than in rats fed with normal diet. These increases were reversed by feeding the plant extracts of Rhus chinensis or Ostrya japonica.
Even though CAT levels in blood serum increased, when rats were fed with ethanol, administration of an antioxidant, ascorbic acid or tocopherol, made the CAT levels drop.
Author(s)
이해원
Issued Date
2011
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000005270
Alternative Author(s)
Lee, Hae Won
Affiliation
제주대학교
Department
대학원 식품공학과
Advisor
고영환
Table Of Contents
Abstract 1

Ⅰ. 항산화성 flavonoid의 neuraminidase 저해 능력 3

1. 서 론 3

2. 재료 및 방법 4
2.1. Bacterial neuraminidase inhibition assay 4
2.1.1. 실험 재료 5
2.1.2. 분석 방법 7
2.2. 파슬리 추출 9
2.3. 총 폴리페놀 함량 분석 9
2.4. 총 플라보노이드 분석 9
2.5. Viral neuraminidase inhibition assay 10
2.5.1. 실험 재료 10
2.5.2. 분석 방법 11
2.6. apigenin 함량 분석 12
2.7. Apigenin 추정 peak의 질량분석 12
2.8. 통계처리 14

3. 결과 및 고찰 14
3.1. Bacterial neuraminidase 활성 저해 14
3.2. 추출 시간에 따른 총 폴리페놀 및 총 플라보노이드 함량 변화 18
3.3. Viral neuraminidase 활성 저해 22
3.4. 파슬리의 apigenin 함량 26
3.5. Apigenin 추정 peak의 질량 확인 30

Ⅱ. 항산화성 천연화합물의 superoxide dismutase, catalase 촉진 능력 33

1. 서 론 33

2. 재료 및 방법 35
2.1. ascorbic acid와 α-tocopherol의 SOD와 CAT 생성에 미치는 영향 35
2.1.1. 실험동물 사육 및 식이 35
2.1.2. 스트레스 유발 및 항산화제의 처리 35
2.1.3. SOD와 CAT의 효소활성 측정 36
2.2. 식물성 항산화 물질의 SOD와 CAT 생성에 미치는 영향 36
2.2.1. 식물 추출물 시료의 제조 36
2.2.2. 스트레스 유발 및 시료의 투여 37
2.2.3. 체중과 장기 무게 측정 37
2.2.4. 장기적출 및 채혈 38
2.2.5. 혈청과 liver homogenate 제조 38
2.2.6. SOD 활성 측정 38
2.2.7. CAT 활성 측정 39
2.2.8. 혈청 화학 검사 39
2.2.9. 통계처리 39

3. 결과 및 고찰 40
3.1. ascorbic acid와 α-tocopherol의 SOD와 CAT 생성에 미치는 영향 40
3.2. 체중의 변화 41
3.3. 간, 비장, 신장의 무게 44
3.4. SOD 활성 48
3.5. CAT 활성 51
3.6. 혈당량 55
3.7. glutamic oxaloacetic transaminase 활성 56
3.8. glutamic pyruvic transaminase 활성 57

Ⅲ. 요약 60

Ⅳ. 참고문헌 62
Degree
Master
Publisher
제주대학교 대학원
Citation
이해원. (2011). 항산화성 천연화합물이 neuraminidase 및 superoxide dismutase외 catalase의 활성에 미치는 영향
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