환경스트레스에 의해 유도되는 크리핑벤트그라스 NAC family 유전자들의 클로닝 및 기능 분석

Abstract

All kinds of crops including foods, feeds, and turf grasses are damaged frequently by various environmental stresses such as drought, salt, and high temperature, which cause the loss of agronomic productivity. Many abiotic stress responsive genes have been identified in plants and they have been applied to breed crops tolerant to environmental stresses through transgenic approach. Creeping bentgrass (Agrostis stolonifera L. cv penncross), commonly used for landscaping of home garden and golf course, is a valuable economic grass. In this study, NAC family genes that seem to be inducible by abiotic stresses were targeted and thus 9 partial cDNAs containing NAC domain sequence were isolated. Their mRNA expressions were responded to abiotic stress such as salt, drought, heat, or dark. From their deduced amino acid comparison, five of AsNAC318, AsNAC319 AsNAC320, AsNAC323, AsNAC324 belonged to the no apical meristem (NAM) subfamily and the remaining four of AsNAC316, AsNAC317, AsNAC321, and AsNAC322 ATAF1. Especially, from three genes of AsNAC316, AsNAC317, and AsNAC322 induced significantly by salt, drought, or dark stress as well as belonging to the ATAF1 subfamily, their full-length cDNAs were cloned by 5' and 3' RACE PCR. AsNAC317 was confirmed to be localized into nucleus by transient assay using onion epidermal cells. Several kinds of binary vectors for plant transformation were constructed to analyze the biological role of the genes in plants. Each AsNAC316, AsNAC317, and AsNAC322 placed under the control of CaMV 35S promoter were transformed into Arabidopsis thaliana.