B16F10 cell 에서 백수오 (Cynanchum wilfordii Radix) 추출물 및 단일화합물의 미백 효능평가

Abstract

Melanogenesis is a well-known physiological response of human skin that exposure to ultraviolet light, for genetic reasons, or due to other causes. therefore In this study, we assessed the effects of C. wilfordii on melanogenic protein expression of murine B16F10 melanoma cells. We obtained an C. wilfordii extract by treatment with 70% MeOH and then successively partitioned it with n-hexane, CH2Cl2, EtOAc, BuOH and water. Present results indicated that the EtOAc fraction of the C. wilfordii extract was effectively inhibited melanogenesis murine B16F10 melanoma cells. Further Vacuum Liquid chromatography on revers phase silicagel separation of this fraction afforded a p-hydroxyacetophenone and cynandione A. Two compounds effect on melanin formation was assessed. Results indicated that cynandione A was shown to down regulate melanin content in a dose-dependent pattern. To clarify the target of cynandione A action in melanogenesis, we performed Western blotting for tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia- associated factor (MITF), which are key melanogenic enzymes. Cynandione A inhibited tyrosinase, TRP-1, TRP-2, and MITF expressions in a dose-dependent manner. Therefore, this study suggests that cynandione A is a candidate anti-melanogenic agent, and it might be effective in hyperpigmentation disorders.