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In vivo and In vitroEffects of Chestnut (Castanea crenata) Inner Shell on Antioxidant and Cardiovascular-Related Parameters

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Author(s)
강민숙
Issued Date
2013
Awarded Date
2013. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006164
Alternative Author(s)
Kang,Min Sook
Affiliation
제주대학교 대학원
Department
대학원 식품영양학과
Advisor
강정숙
Table Of Contents
INTRODUCTION 1
LITERATURE REVIEW 4
1.Risk factors of cardiovascular disease 4
1)Atherosclerosis 4
2)Oxidative stress and inflammation in atherosclerosis 6
3)Hypertension 9
2.Prevention properties of polyphenols on cardiovascular disease 11
3.Potential possibility of utilization of chestnut inner shell as bio-resource 13

Part I. In vivo study for the effects of chestnut (Castanea crenata) inner shell on antioxidant and cardiovascular-related parameters

ABSTRACT 17
MATERIALS AND METHODS 19
1.Materials 19
1)Animals and diets 19
2)Preparation of chestnut inner shell extract 19
2.Collection of samples 21
3.Sample analysis 21
1)Whole blood platelet aggregation 21
2)Plasma and liver lipid assay 22
3)Platelet rich plasma (PRP) and liver TBARS productions 22
4)Erythrocyte Na efflux channels 23
5)Plasma GOT and GPT levels 27
6)Lung angiotensin converting enzyme (ACE) activity 28
4.Statistical analysis 28
RESULTS 30
1.Body weights, plasma and liver lipids 30
2.Hematocrit and whole blood platelet aggregation 33
3.Plasma GOT and GPT levels 35
4.Erythrocyte Na efflux and ACEinhibitory effect 37
5.Erythrocyte Na-leak 39
6.Platelet rich plasma (PRP) and liver TBARS productions 41
DISCUSSION 43

Part II. In vitro study for the effects of chestnut (Castanea crenata) inner shell on antioxidant and cardiovascular-related parameters

ABSTRACT 50
MATERIALS AND METHODS 52
1.Materials 52
1)Chemical materials 52
2)Solvent fractionation of chestnut inner shell extract 52
3)Blood and lung samples preparation 53
4)Lymphocyte isolation 54
2.Methods 54
1)Free radical scavenging activity 54
2)Determination of total polyphenolic content 56
3)Comet assay for determination of DNA damage 57
4)Angiotensin converting enzyme (ACE) inhibition activity 58
5)Whole blood platelet aggregation 59
6)Assay for pro-inflammatory mediators 60
3.Statistical analysis 63
RESULTS 64
1.Free radical scavenging activities of Ech and the major polyphenols in Ech 64
2.DPPH radical scavenging activities and total polyphenolic contents of Ech and its solvent fractions 68
3.Protective effect against H2O2-induced DNA damage 70
4.Angiotensin converting enzyme (ACE) inhibition activity 72
5.Whole blood platelet aggregation 74
6.Effects on NO production in LPS-stimulated RAW 264.7 cells 76
7.Effects on PGE2 production in LPS-stimulated RAW 264.7 cells 78
8.Effects on protein levels of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells 80
9.Effects on the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells 82
DISCUSSION 86

REFERENCE 93
Degree
Doctor
Publisher
제주대학교
Citation
강민숙. (2013). In vivo and In vitroEffects of Chestnut (Castanea crenata) Inner Shell on Antioxidant and Cardiovascular-Related Parameters
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General Graduate School > Food Science and Nutrition
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