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Subcritical Water Hydrolysis of Rutin into Isoquercetin and Quercetin

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Abstract
Maximum production of isoquercetin and quercetin simultaneously from rutin by subcritical water hydrolysis (SWH) was optimized using the response surface methodology. Hydrolysis parameters such as temperature, time, and CO2 pressure were selected as independent variables, and isoquercetin and quercetin yields were selected as dependent variables. The regression models of the yield of isoquercetin and quercetin were valid due to the high F-value and low p-value. Furthermore, the high regression coefficient indicated that the polynomial model equation provides a good approximation of experimental results. In maximum production of isoquercetin from rutin, the hydrolysis temperature was the major factor, and the temperature or time can be lower if the CO2 pressure was increased high enough, thereby preventing the degradation of isoquercetin into quercetin. The yield of quercetin was considerably influenced by temperature instead of time and CO2 pressure. The optimal condition for maximum production of isoquercetin and quercetin simultaneously was temperature of 171.4°C, time of 10.0 min, and CO2 pressure of 11.0 MPa, where the predicted maximum yields of isoquercetin and quercetin were 13.7% and 53.3%, respectively. Hydrolysis temperature, time, and CO2 pressure for maximum production of isoquercetin were lower than those of quercetin. Thermal degradation products such as protocatechuic acid and 2,5-dihydroxyacetophenone were observed due to pyrolysis at high temperature. It was concluded that rutin can be easily converted into isoquercetin and quercetin by SWH under CO2 pressure, and this result can be applied for SWH of rutin-rich foodstuffs.
Rutin을 아임계수로 처리 온도, 시간, 이산화탄소 압력을 독립변수로 선정하여 반응표면분석법을 이용하여 isoquercetin과 quercetin으로의 가수분해 조건을 최적화하였다. Rutin의 isoquercetin과 quercetin로의 전환에 대한 반응표면 모델은 높은 F-value와 낮은 p-value, 그리고 높은 회귀계수로 인하여 적합하였다. Isoquercetin의 생성을 위해서는 가수분해 온도가 주요 인자이었으며, 이산화탄소의 압력을 증가시키면 가수분해 온도와 시간을 낮출 수 있었으며 고온과 장시간 처리에 따른 isoquercetin의 파괴를 방지할 수 있었다. Quercetin의 생성은 가수분해 시간과 이산화탄소 압력보다 가수분해 온도에 의해 큰 영향을 받았다. Isoquerceitn과 quercetin을 동시에 최대로 생성할 수 있는 최적조건은 온도 171.4℃, 시간 10.0분, 이산화탄소 압력 11.0 MPa이었으며, 이때의 반응표면 모델에 의하여 예측된 수율은 각각 13.7%와 53.3%이었다. Isoquercetin의 최대 생성을 위한 가수분해 온도, 시간과 이산화탄소 압력은 각각 168.0℃, 11.1분, 8.6 MPa로서 quercetin의 최적 조건인 179.8℃, 22.5분, 14.7 MPa 보다 모두 낮았다. 고온 처리에 의한 열분해 생성물로는 protocatechuic acid, 2,5-dihydroxy acetophenone 등과 같은 화합물이 검출되었다. 이상의 결과로부터 rutin은 이산화탄소 첨가 하에서 아임계수 가수분해에 의하여 isoquercetin과 quercetin으로 쉽게 전환될 수 있었으며, 본 연구의 결과는 루틴이 다량 함유되어 있는 식품 원료에서 isoquercetin과 quercetin을 생산하는데 유용하게 적용될 수 있을 것으로 추정되었다.
Author(s)
김동신
Issued Date
2017
Awarded Date
2017. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000008157
Alternative Author(s)
Kim, Dong Shin
Affiliation
제주대학교 일반대학원
Department
대학원 식품공학과
Advisor
임상빈
Table Of Contents
Abstract 1
1. Introduction 2
2. Materials and methods
2.1. Chemicals 5
2.2. Experimental design 5
2.3. Subcritical water hydrolysis 7
2.4. HPLC analysis 9
2.5. Calculation of isoquercetin and quercetin yields, and rutin loss 10
2.6. GC/MS identification of degradation products 11
2.7. Statistical analysis 13
3. Results and discussion
3.1. Fitting of the response surface models 13
3.2. Effect of the hydrolysis parameters on the yield of isoquercetin 17
3.3. Effect of the hydrolysis parameters on the yield of quercetin 20
3.4. Optimization conditions for maximum yields of isoquercetin and quercetin 21
3.5. Verification of the optimal conditions 23
3.6. GC/MS identification of degradation products 25
국문요약 27
References 28
Degree
Master
Publisher
제주대학교 일반대학원
Citation
김동신. (2017). Subcritical Water Hydrolysis of Rutin into Isoquercetin and Quercetin
Appears in Collections:
General Graduate School > Food science and Engineering
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