항산화제 헤스페레틴이 돼지 난자의 노화기간 동안 난자의 미치는 영향 연구

Abstract

The citrus flavonoid hesperetin has a variety of pharmacological actions, including antioxidant, anti-inflammatory, and anticancer activities. This study investigated whether hesperetin prevents aging of oocytes in vitro in which it determined the maturation of nuclear and cytoplasm and the developmental capacity of embryo by modulating the ROS level. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 1, 10, 100, and 250 μM Hhesperetin (aging, H-1, H-10, H-100, and H-250, respectively). Although there was no difference in the rate of maturation among all the groups, both the control H-100 groups significantly increased in the rate of cleavage and blastocyst formation compared to the aging group (p < 0.01). The H-100 group significantly decreased reactive oxygen species (ROS) activity and increases the level of glutathione (GSH) and expression of the antioxidant genes (PRDX5, NFE2L, SOD1 and SOD2) compared to the aging group. The H-100 groups prevented aberrant spindle organization and chromosomal misalignment, blocked the decrease in the level of phosphorylated -p44/42 mitogen -activated protein kinase (MAPK) and increased the mRNA expression of cytoplasmic maturation factor genes (GDF9, CCNB1, BMP15 and MOS). Subsequently, both the control and H-100 groups significantly increased the total cell number and decreased the apoptosis cells at blastocyst stage compared to aging group. The results indicate that hesperetin improves the quality of porcine oocytes by protecting them against oxidative stress during aging in vitro.