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Antioxidant Potential of Sargassum horneri Ethanol Extract against Urban Particulate Matter Induced Oxidation

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Abstract
Sargassum horneri is a brown seaweed found in the coast of East Asia. Particulate matter, a major contribution of air pollution, has caused detrimental effect on human health. In this study, in vitro antioxidant activities of Sargassum horneri ethanol extract (SHE) against particulate matter induced oxidative stress were investigated by measuring total phenolic contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, hydrogen peroxide (H2O2) scavenging, superoxide anion (O2 .-) inhibition rate, hydroxyl radical (∙OH) scavenging, metal chelating effect, and reducing power. Also the identification and quantification of phenolic compounds were carried out using high performance liquid chromatography. Consequently, gallic acid (10.57 mg/g) was the predominant polyphenol compound in SHE. All in vitro antioxidant activities were increased as the concentration of SHE increased (0-1000 μg/mL). Especially, the DPPH free radical, H2O2, O2 .- and ∙OH scavenging activity, and metal ion chelating effect of SHE at 1000 μ g/mL was 69.90, 95.99, 69.25, 38.48, and 67.02%, respectively, which exhibited the highest activities. When treated with particulate matter at 0 to 1000 μ g/mL along with Sargassum horneri ethanol extract at 62.5, 125, and 250 μ g/mL, the DPPH free radical, H2O2, metal ion chelating, and reducing power were significantly reduced compared to the sample without SHE (p<0.05). These results indicate that Sargassum horneri ethanol extract effectively suppressed the oxidative stress induced by particulate matter.
본 연구에서는 미세먼지 유도 산화적 스트레스에 대한 농도별 괭생이 모자반 (Sargassum horneri) 에탄올 추출물의 in vitro 항산화 활성성분을 알아보기 위 해 총 폴리페놀 함량, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical 소거활 성, hydrogen peroxide (H2O2) 소거활성, superoxide anion radical (O2 .-) 소거활 성, hydroxyl radical (∙OH) 소거활성, 금속 킬레이트, 환원력을 측정 비교하였 다. 또한 총 11 종의 페놀 화합물 함량 분석은 HPLC-DAD로 정량한 결과, 괭생 이 모자반 에탄올 추출물에서는 gallic acid의 함량이 10.57 mg/g으로 가장 높았 으며, 총 폴리페놀 함량 또한 67.58 mg GAE/g으로 높게 측정되었다. 괭생이 모 자반 에탄올 추출물에서 모든 in vitro 항산화능을 측정한 결과 추출물의 농도가 높을수록 그 효과가 증가하였으며, 특히 추출물의 농도 1000 μg/mL일 때 DPPH free radical 소거활성, hydrogen peroxide 소거활성, superoxide anion radical 소 거활성, hydroxyl radical 소거활성, 금속 킬레이트에서 각각 69.90, 95.99, 69.25, 38.48, 67.02%로 radical 소거능이 강했다. 반대로 괭생이 모자반 에탄올 추출물 의 농도가 62.5, 125, 250 μg/mL일 때, 미세먼지의 농도를 0-1000 μg/mL로 처리 하였을 경우 추출물의 DPPH free radical 소거활성, hydrogen peroxide 소거활 성, 금속 킬레이트, 환원력 평가 결과에서는 미세먼지의 첨가 농도가 증가할수록 항산화활성이 감소하는 경향을 보였다. 이는 괭생이 모자반 에탄올 추출물에 처 리된 미세먼지가 영향을 미쳤을 것이라 판단된다. 그럼에도 불구하고 괭생이 모 자반 에탄올 추출물은 높은 라디칼 소거능과 항산화 활성을 보여주었으므로 괭 생이 모자반은 항산화 활성을 나타내는 기능성 식품소재로의 이용 가능성이 높 을 것으로 기대된다.
Author(s)
이주희
Issued Date
2019
Awarded Date
2019. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/common/orgView/000000008897
Alternative Author(s)
Lee, Ju Hee
Affiliation
제주대학교 대학원
Department
대학원 식품공학과
Advisor
김현정
Table Of Contents
Abstract 1
1. Introduction 2
2. Materials and methods
2.1. Materials and chemicals 5
2.2. Preparation of Sargassum horneri ethanol extract 6
2.3. Determination of total phenolic content 7
2.4. Analysis of phenolic compounds by HPLC-DAD system 8
2.5. DPPH free radical scavenging activity 9
2.6. Hydrogen peroxide scavenging activity 10
2.7. Superoxide anion scavenging activity 11
2.8. Hydroxyl radical scavenging activity 12
2.9. Metal ion chelating effect 13
2.10. Reducing power 14
2.11. Statistical analysis 15
3. Results and discussion
3.1. Total phenolic content 16
3.2. Phenolic compounds by HPLC 17
3.3. DPPH free radical scavenging activity 19
3.4. Hydrogen peroxide scavenging acitivity 22
3.5. Superoxide anion scavenging activity 26
3.6. Hydroxyl radical scavenging activity 28
3.7. Metal ion chelating effect 31
3.8. Reducing power 35
3.9. In vitro antioxidant activities 39
4. Conclusion 42
국문요약 44
References 45
Degree
Master
Publisher
제주대학교 대학원
Citation
이주희. (2019). Antioxidant Potential of Sargassum horneri Ethanol Extract against Urban Particulate Matter Induced Oxidation
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General Graduate School > Food science and Engineering
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