The neuroprotective effects of Archidendron jiringa(Jack)I.C.Nielsen extract inhibit glutamate-induced apoptosis in hippocampal HT22 cells.
- Archidendron Jiringa is a leguminous tree growing mainly in Southeast Asia. A recent study found that A. jiringa has the effect of anticancer, anti-inflammatory, anti-diabetic and insect repellents. However, the neuroprotective effect of the A. jiringa extract on glutamate induced cell apoptosis was not studied. Glutamate activates the formation of reactive oxygen species(ROS) and the pathways of the mitogen-activated protein kinase (MAPK). MAPK is the oxidative stress pathway, and simultaneously regulates Calpain 1, Caspase 12, Bax, Bcl-2 and AIF which are the Endoplasmic Reticulum(ER) stress pathway. In this study, the effects of A. jiringa extract on glutamate induced oxidative stress and ER stress in HT22 mouse hippocampal cells were investigated.
It has been found that pretreatment of A. jiringa extract reduces ROS production and recovers HT22 cells from glutamate induced cell apoptosis. In addition, our study confirmed that A. jiringa extract inhibited MAP kinase signaling pathway, activated Calpain 1, Caspase 12, Bax, AIF and inhibited Bcl-2. Our results suggest that the A. jiringa extract prevents damage from glutamate induced oxidative stress and ER stress. It provides experimental evidence that it can be used as a potential phytochemical for the treatment and prevention of neurodegenerative diseases due to the neuroprotective effects of A. jiringa.
- Lee, Cheol
- Issued Date
- Awarded Date
- 2020. 8
- Alternative Author(s)
- 제주대학교 대학원
- 대학원 생명공학과
- Table Of Contents
- CONTENTS i
LIST OF FIGURES ii
1. INTRODUCTION 2
2. MATERIALS AND METHODS 5
2.1. DPPH radical scavenging assay 5
2.2. Cell culture and reagents. 5
2.3. Preparation of sample 5
2.4. Cell viability assay 5
2.5. Flow cytometry analysis 6
2.6. Measurement of intracellular ROS. 6
2.7. Western blot assay 6
2.8. Statistical analysis. 7
3. RESULTS . 8
3.1. Subtropical natural product screening for radical scavenging 8
3.2. A. jiringa extract protects glutamate-induced HT22 neuronal cell death. 10
3.3. A. jiringa extract reverses glutamate-induced apoptotic HT22 cell death. 12
3.4. A. jiringa extract inhibits glutamate-induced ROS production 13
3.5. A. jiringa extract regulates signal pathway related MAPK and ER stress. 15
4. DISCUSSION. 17
5. REFERENCES. 20
- 제주대학교 대학원
- Lee, Cheol. (2020). The neuroprotective effects of Archidendron jiringa(Jack)I.C.Nielsen extract inhibit glutamate-induced apoptosis in hippocampal HT22 cells.
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