Neuroprotective effects of Carpinus tschonoskii leaves extract against glutamate-induced oxidative stress in HT22 hippocampal cells

Abstract

The present study investigated the neuroprotective effect of the intracellular protection mechanism using C.tschonoskii leaves extract (CTE) against glutamate-induced oxidative damage in HT22 cells. We found that pretreatment of HT22 cells with CTE significantly inhibited the cell death induced by glutamate in a dose dependent manner. CTE reduced ROS production and apoptosis induced by glutamate. In addition, CTE inhibited phosphorylation of p38 and Erk, transcription factors important for neuronal survival, and reduced the expression of Bax and AIF, apoptosis-related proteins. The result is that CTE in HT22 cells can prevent glutamate-induced apoptosis through ROS production, phosphorylation inhibition of oxidative stress related proteins, and reduction of expression. Therefore, it suggests that it can be applied to the development of therapeutic agents for diseases of the brain and nervous system.