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Synthesis, Characterizations and Implementation of Functional Nanostructured Materials and Composites for Point-Of-Care Testing Devices

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Abstract
The emerging field of nanotechnology owes the capability to develop highly functional and novel nanostructured materials (NSMs), which have been addressing challenging applications due to its abundant strategic properties. NSMs have gained notability in diverse fields from the last two decades due to their tunable and unique physical, chemical, and biological properties. In the field of biosensors, NSMs have been providing the desirable properties by virtue of their significance in detection and analysis methods. This research work focuses on the synthesis, bioconjugation, functionalization, and characterization of NSMs and the development of high-performance biosensor devices targeting point-of-care-devices for the detection of analytes (Uric acid, Lactate, Vaspin, and Reactive oxygen species). In this thesis, we are focusing on the synthesis of Zero dimensional (OD) nanoparticles such as ZnO Quantum dots (ZnO QDs), Platinum nanoparticles (PtNPs) and upconverting nanoparticles (UCNPs) conjugation, one-dimensional (1D), nanotubes (multiwalled carbon-based) and two-dimensional (2D), multiwalled carbon nanotubes/poly (3,4-ethylene-dioxythiophene) poly(styrene sulfonate) (MWCNTs/PEDOT:PSS) composite. Moreover, biofunctionalization of UCNPs and bioconjugation with aptamers has been carried out. The characterization of the developed nanomaterials has been carried out using HR-TEM, FE-SEM, AFM, XRD, Raman, FTIR, EDX, and UV/VIS spectroscopy. These tailored nanostructured materials are implemented in the fabrication of electrochemical and optical-based biosensor devices for the detection of the requisite metabolites/biomarkers. These nanostructured materials have provided a steady platform for the immobilization of bioreceptors with biocompatibility, electrocatalytic properties along with high sensitivity, an improved range of detection (ROD), and detection limit (LOD). In brief, the implementation of NSMs has shown excellent performance in biosensing.
Author(s)
Muhsin Ali
Issued Date
2021
Awarded Date
2021. 2
Type
Dissertation
URI
https://oak.jejunu.ac.kr/handle/2020.oak/23505
Alternative Author(s)
무신 알리
Affiliation
Graduate School of Jeju National University
Department
대학원 에너지응용시스템학부 Mechatronics Engineering
Advisor
Choi, Kyung Hyun
Table Of Contents
1. Introduction 1
1.1. Biomarkers 1
1.2. Nanostructured materials 1
1.2.1. Quantum dots 2
1.2.2. Nanowires 2
1.2.3. Nanocomposites 2
1.2.4. Nanocubes 3
1.3. Biosensors 3
1.3.1. Electrochemical biosensors 4
1.3.2. Optical biosensors 4
1.3.3. Mass based biosensors 5
1.4. POCT (Point-of-care) devices 5
2. Chapter 2. Electrochemical biosensors 6
2.1. Electrochemical based detection of Uric Acid through ZnO QDs guiding layer 6
2.1.1. Background and overview 6
2.1.2. ZnO quantum Dots synthesis 8
2.1.3. Screen printed biosensor fabrication 8
2.1.4. Characterizations of ZnO QDs 10
2.1.5. Results 12
2.1.6. Application: detection of UA 16
2.2. Electrochemical based detection of lactate through novel composite (MWCNTs/PEDOT:PSS) 17
2.2.1. Background 17
2.2.2. Nanocomposite synthesis and electrode functionalization 19
2.2.3. Composite characterizations 21
2.2.4. Optimization of analytical parameters 25
2.2.5. Analytical performance of the sensor 26
2.2.6. Application: lactate detection in cancer cells media 29
3. Chapter 3. Fluorescent based biosensor 32
3.1. Upconverting nanoparticles based vaspin biosensors strip overview 32
3.1.1. Nanoparticles (NayF4: Yb, Er) size reduction 33
3.1.2. Functional group exchange Mal-PEG-COOH 34
3.1.3. Bioconjugation of aptamers 34
3.1.4. Lateral flow strip 36
3.1.5. Sample assay 37
3.1.6. Characterizations of conjugated nanoparticles 39
3.1.7. Vaspin detection using LFSA strip in buffer samples 43
3.1.8. Application: Vaspin detection in serum samples 45
3.2. Fluorescent dye based detection of Reactive oxygen Species (ROS) in microfluidic chip 46
3.2.1. Background 47
3.2.2. Optimization of concentration and mechanism of dye interaction with cells 50
3.2.3. Microfluidic chip 51
3.2.4. Cell culture on a microfluidic chip 51
3.2.5. In-house built fluorescence microscope 53
3.2.6. Image processing 54
3.2.7. Results and Discussion 54
3.2.8. Application: Real time monitoring and evaluation of ROS in breast cancer cells line (MCF-7) 63
4. Chapter 4. Colorimetric based biosensor 67
4.1. Colorimetric based Uric acid detection (non-enzymatic) 67
4.2. Citrate capped Pt nanoparticles (PtNPs) synthesis 69
4.3. Non-enzymatic based UA detection 70
4.4. Design of POCT device 71
4.5. Characterizations of PtNPs 73
4.6. Colorimetric sensing mechanism 75
4.7. Theoretical calculations 78
4.8. Detection of Uric Acid on cellulose strip 79
4.9. Application: Detection of UA in urine samples 82
5. Conclusion and future work 85
References 89
Degree
Doctor
Publisher
Graduate School of Jeju National University
Citation
Muhsin Ali. (2021). Synthesis, Characterizations and Implementation of Functional Nanostructured Materials and Composites for Point-Of-Care Testing Devices
Appears in Collections:
Faculty of Applied Energy System > Mechatronics Engineering
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