Tocopheryl Acetate를 함유한 Nanoemulsion의 피부흡수 연구

Abstract

피부는 표피, 진피 및 피하 지방의 세 부분으로 나눌 수 있으며, 표피의 각질층은 약물 전달을 방해하는 장벽 역할을 한다. 나노에멀젼은 특유의 작은 입자 크기 때문에 세포 간 지질을 통해 약물의 경피 전달에 효과적인 것으로 알려져 있다. 이 연구에서는 Tocopheryl acetate의 효과적인 피부 흡수를 위해 고에너지 및 저에너지 방법에 의해 제조된 나노에멀젼을 반응표면분석법을 사용하여 최적화하였다. 예비 실험으로서, 요인배치법을 수행하였고, 요인배치법의 결과를 바탕으로 고에너지 방법은 계면활성제 함량(3.0 ~ 9.0 wt), 고압 유화 압력(600 ~ 1,000bar), 고압 유화 횟수(5 ~ 13pass)를 인자로 하였고, 저에너지 방법에서는 보조계면활성제 함량(5.2 ∼7.8 wt%), 오일 함량(13.0 ~ 23.0 wt%), 유화 온도(75 ~ 95°C)를 인자로 하였다. 나노에멀젼 입자 크기 및 다분산도를 반응변수로 하여 Box-Behnken design을 수행하였다. 반응표면분석법 결과에 따라 PIT 및 HPH 나노에멀젼을 최적화한 후, 두 나노에멀젼의 피부 흡수율을 확인하였다. 그 결과, PIT 나노에멀젼의 피부흡수율은 3.84 ± 0.26%, HPH 나노에멀젼의 피부흡수율은 8.91±1.57%인 것을 확인하였다.

The skin is divided into three parts: the epidermis, the dermis, and the subcutaneous fat, and the stratum corneum, which is located at the top of the epidermis, acts as a barrier that prevents drug delivery. Nanoemulsions are known to be effective in transdermal delivery of drugs through intercellular lipids because of their unique small particle size. In this study, for effective skin absorption of Vitamin E Acetate, nanoemulsion prepared by high-energy and low-energy methods was optimized using response surface methodology. As a preliminary experiment, frational factorial design and full factorial design were performed. Based on the result of the factorial design, the high energy method used the surfactant content (3.0 ∼ 9.0 wt%), high pressure emulsification pressure (600 ∼ 1,000 bar), and the number of high pressure emulsification (5 ∼ 13 pass) as factors and the low energy method was the cosurfactant content (5.2 ∼ 7.8 wt%), oil content (13.0 ∼ 23.0 wt%), and emulsification temperature (75 ~ 95℃) were used as factors. A Box-Behnken design was performed with the reaction variable as the particle size of the nanoemulsion. HPH and PIT nanoemulsion were optimized according to the response surface methodology results, and skin absorption rates of the two nanoemulsions were compared. As a result, the final skin absorption rate was 3.84 ± 0.26% of PIT nanoemulsion and 8.91 ± 1.57% of HPH nanoemulsion, confirming that the skin absorption rate of HPH nanoemulsion was better.