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화이트 클로버(Trifolium repens L.) 모본과 교배종의 외부형태학적 분류 및 분자마커 개발

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Abstract
White clover (Trifolium repens L.) is an herbaceous perennial plant of the Fabaceae family. It is native
in Europe and Central Asia and now globally cultivated as a forage crop. For centuries, white clover
has been widely used as a feed for livestock due to its high protein content or as a cover crop due to its
spreading properties. In addition, due to characteristics such as color and pattern of leaves, it has
excellent potential as an ornamental plant. In this study, I classified 5 types of parental clover (Ladino,
Green ice, William, Pentaphyllum and Dragon’s blood) and 6 types of hybrids clover [JH-1(Ladino x
Green ice (Overlap)), JH-2 [Ladino x Green ice (White V mark)], JH-3 (Ladino x Dragon’s blood), JH-
4 (Pentaphyllum x William), JH-5 (William x Dragon’s blood) and JH-6 (Pentaphyllum x Dragon’s
blood)) with a 11 Morphological traits such as plant form, leaf shape, leaf edge shape, upper lamina
color, lower lamina color, white V mark, midrib color, Front side of leaf pattern, Back side of leaf
pattern, flower color and peduncle color. After the morphological classification, I also developed RAPD
(Random Amplified Polymorphic DNA) and SCAR (Sequence characterized ampified region) markers
to classify the parental and hybrids of white clover at the molecular level. In the RAPD analysis, 13 of
the RAPD primers (OPC-13, OPD-06, OPE-08, OPE-10, OPE-18, OPG-04, OPG-12, OPH-02, OPH-
16, OPH-11, OPI-16, OPJ-12 and OPK-01) were found to exhibit genetic polymorphism between the
parental and the hybrids of white clover. In this study, RAPD polymorphism bands showing all the
polymorphisms identified in the two parent breeds in the hybrid were converted to SCAR primers by
performing sequencing of polymorphic bands. As a result, only about 26% of 38 SCAR primers were
converted to SCAR markers. In this study, it was demonstrated that the combination of 11
morphological traits, RAPD and SCAR markers can be used to identify varieties of white clover. It is
also expected to be usefully used for breeding and identification of new varieties of white clover in the
future.
Author(s)
한승섭
Issued Date
2023
Awarded Date
2023-02
Type
Dissertation
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000011020
Alternative Author(s)
Han, Seong Seop
Affiliation
제주대학교 대학원
Department
대학원 분자생명공학전공
Advisor
이효연
Table Of Contents
Ⅰ. 서론 1
Ⅱ. 재료 및 방법 5
1. 식물재료 5
2. 화이트 클로버 선발 7
3. Genomic DNA 추출 9
4. RAPD 분석 10
5. 선발된 RAPD마커의 SCAR마커로의 전환 14
Ⅲ. 결과 및 고찰 16
1. 화이트 클로버의 외부형태학적 분류 16
1-1. 화이트 클로버 모본의 외부형태학적 분류 17
1-1-1. Ladino 17
1-1-2. Green ice 18
1-1-3. William 19
1-1-4. Pentaphyllum 20
1-1-5. Dragon's blood 21
1-2. 화이트 클로버 교배종의 외부형태학적 분류 23
1-2-1. JH-1(Ladino x Green ice) 23
1-2-2. JH-2(Ladino x Green ice) 25
1-2-3. JH-3(Ladino x Dragon's blood)- 26
1-2-4. JH-4(William x Pentaphyllum) 27
1-2-5. JH-5(William x Dragon's blood) 28
1-2-6. JH-6(Pentaphyllum x Dragon's blood) 29
2. RAPD분석을 이용한 모본 및 교배종 화이트클로버의 유연관계 분석 30
2-1. JH-1 and JH-2(Ladino x Green ice) 30
2-2. JH-3(Ladino x Dragon's blood) 35
2-3. JH-4(William x Pentaphyllum) 37
2-4. JH-5(William x Dragon's blood) 41
2-5. JH-6(Pentaphyllum x Dragon's blood) 45
3. 화이트 클로버 품종 선별을 위한 SCAR 마커 개발 50
3-1. JH-1 and JH-2(Ladino x Green ice) 53
3-2. JH-4(William x Pentaphyllum) 56
3-3. JH-5(William x Dragon's blood) 59
3-4. JH-6(Pentaphyllum x Dragon's blood 62
Ⅳ. 종합 고찰 65
Ⅴ. 참고 문헌 68
Degree
Master
Publisher
제주대학교 대학원
Citation
한승섭. (2023). 화이트 클로버(Trifolium repens L.) 모본과 교배종의 외부형태학적 분류 및 분자마커 개발.
Appears in Collections:
Faculty of Biotechnology > Molecular Biotechnology
공개 및 라이선스
  • 공개 구분공개
  • 엠바고2023-02-17
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