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Photoprotective Effects of Germinated and Fermented Soybean Extract on UVB-irradiated SKH-1 Hairless Mice

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Alternative Title
광노화 유발된 피부에 대한 발아발효콩 추출물의 개선 효과
Abstract
Ultraviolet (UV) radiation is a primary contributor to skin aging, accounting for approximately 90% of signs of premature aging. Continuous ultraviolet irradiation rapidly induces a large accumulation of reactive oxygen species (ROS) in the body, causing various metabolic diseases and causing direct damage to skin cells due to oxidative stress. The phytochemical isoflavones present in soybeans are recognized for exerting physiological effects, including anti-tumor, anti-obesity, and especially anti-aging properties. However, there is currently limited research on phytoestrogens, including the ability of phytochemicals to improve skin aging functions. This study was to evaluate the anti-inflammatory and potent antioxidant properties of germinated and fermented soybean extract (GFSE) in improving photoaging induced by continuous UVB irradiation in hairless mouse. Photoaging was induced in hairless mice by irradiating UVB for 8 weeks, and GFSE was orally administered daily (250 and 500 mg/kg). The skin was collected and parameters and wrinkle formation analysis and histopathological analysis were performed to indirectly assess the effects on skin conditions. Additionally, skin hyaluronic acid (HA) content, protein expression, and mRNA were evaluated for the photoprotective effects of GFSE through molecular biological approaches. GFSE significantly improved UVB-induced skin parameters and wrinkle index and reduced the increased thickness of the epidermis. In addition, the HA content reduced by UVB was increased. GFSE decreased the expression of matrix metalloproteinases, which were increased by UVB, and increased tissue metallopeptidase inhibitors and collagen type 1α1, which were decreased by UVB. It also increased antioxidant-related factors such as superoxide dismutase 1, catalase, and glutathione peroxidase, and increased factors related to the skin barrier and selective skin moisture absorption, such as involucrin, filaggrin, and aquaporin 3. GFSE administration reduced interleukin-1β, cyclooxygenase 2, and tumor necrosis factor-α which are inflammatory cytokines produced due to photoaging. Moreover, UVB reduced phosphorylated protein levels of Jun N-terminal kinase, extracellular signaling regulatory kinase, and p38, as well as cyclooxygenase 2 and inducible nitric oxide synthase protein levels.
GFSE significantly improved skin photoaging and reduced the extent of skin damage in hairless mice exposed to UVB irradiation. These findings suggest that GFSE may be a functional food ingredient for alleviating skin photoaging.
Author(s)
천가영
Issued Date
2024
Awarded Date
2024-02
Type
Dissertation
URI
https://dcoll.jejunu.ac.kr/common/orgView/000000011863
Alternative Author(s)
Cheon Ga yeong
Affiliation
제주대학교 대학원
Department
대학원 식품영양학과
Advisor
홍기배
Table Of Contents
1. Introduction 1
2. Materials and methods 12
2.1 GFSE sample preparation 12
2.2 Experimental animal 14
2.3 UVB-irradiated photoaging model 15
2.4 Evaluation of skin parameters 15
2.5 Evaluation of wrinkle formation 15
2.6 Histopathological analysis of the skin 16
2.7 Analysis of hyaluronic acid content (HA) in skin tissue 16
2.8 Analysis of gene expression through qRT-PCR 17
2.9 Western blot analysis 19
2.10 Statistical analyses 19
3. Results 20
3.1 Changes in physical condition of UVB-irradiated hairless mice 20
3.2 Measurement of organ weights in UVB-irradiated hairless mice 22
3.3 Effects of GFSE on skin parameters in UVB-irradiated hairless mice 24
3.4 Effects of GFSE on UVB-induced skin wrinkle formation in UVB-irradiated hairless mice 26
3.5 Effects of GFSE on epidermal thickness in UVB-irradiated hairless mice 28
3.6 Effects of GFSE on skin HA content in UVB-irradiated hairless mice 30
3.7 Effects of GFSE on collagen homeostasis-related mRNA expression in UVB- irradiated hairless mice 32
3.8 Effects of GFSE on antioxidant enzyme-related mRNA expression in UVB- irradiated hairless mice 35
3.9 Effects of GFSE on skin barrier function-related mRNA expression in UVB- irradiated hairless mice 37
3.10 Effects of GFSE on skin inflammation-related mRNA expression in UVB- irradiated hairless mice 39
3.11 Effects of GFSE on MAPK pathway-related protein levels in UVB-irradiated hairless mice 41
3.12 Effects of GFSE on skin inflammation-related protein levels in UVB-irradiated hairless mice 43
4. Discussion 45
5. Conclusion 50
6. References 51
국문 초록 57
Degree
Master
Publisher
제주대학교 대학원
Citation
천가영. (2024). Photoprotective Effects of Germinated and Fermented Soybean Extract on UVB-irradiated SKH-1 Hairless Mice.
Appears in Collections:
General Graduate School > Food Science and Nutrition
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