흰점박이꽃무지 유충의 건조 및 단백가수분해물의 특성

Abstract

This study was conducted to prepare a powder and protein hydrolysate from live Protaetia brevitarsis larva. In order to prepare the Protaetia brevitarsis larva powder, the manufacturing condition was variously designed with sacrifice method (blanching or freezing at –80℃), defatting process (defat, non-defat), storage temperature (-20℃, -80℃) and drying method (hot-air drying, freeze drying). And then we evaluated the appearance, yield, moisture contents, pH, color, proximate analysis, volatile basic nitrogen, DPPH free radical scavenging activity and total phenol contents of Protaetia brevitarsis larva powder. The optimal condition was the combination of blanching, defatting and -20℃ storage temperature and hot-air drying processes. When blanching the Protaetia brevitarsis larva, water contents and volatile basic nitrogen of them were low. When defatting, the lightness and total phenol contents increased. When hot air dried, the crude fat contents were lower than that of freeze drying. It was suggested that in a large scale industries, the optimal condition combination(blanching, defatting, hot-air drying) to make a powder is more cost-effective and maintaining the quality of Protaetia brevitarsis larva powder. To produce Protaetia brevitarsis larva hydrolysate, the Protaetia brevitarsis larva powder manufactured by optimal processing was hydrolyzed using ultrasound treatment and enzymes (alcalase and neutrase). The appearance, yield, pH, degree of hydrolysis, solubility, SDS-PAGE, antioxidant activity (DPPH free radical scavenging activity, FRAP assay, ABTS radical scavenging, Hydrogen peroxide scavenging) of the hydrolysates were evaluated. Ultrasound treatment was effective to increase antioxidant activity and solubility of hydrolysate at two type enzymatic hydrolysis. In the alcalase hydrolysis, the initial hydrolysis rate also increased rapidly, and hydrolysate by alcalase were higher antioxidant activity than neutrase. During alcalase hydrolysis, it was observed that a low molecular weight peptide was produced according to the hydrolysis time. In this study, it was observed that bioactive peptides can be produced effectively by alcalase enzymatic hydrolysis and it was better utility when ultrasound assisted.

This study was conducted to prepare a powder and protein hydrolysate from live Protaetia brevitarsis larva. In order to prepare the Protaetia brevitarsis larva powder, the manufacturing condition was variously designed with sacrifice method (blanching or freezing at –80℃), defatting process (defat, non-defat), storage temperature (-20℃, -80℃) and drying method (hot-air drying, freeze drying). And then we evaluated the appearance, yield, moisture contents, pH, color, proximate analysis, volatile basic nitrogen, DPPH free radical scavenging activity and total phenol contents of Protaetia brevitarsis larva powder. The optimal condition was the combination of blanching, defatting and -20℃ storage temperature and hot-air drying processes. When blanching the Protaetia brevitarsis larva, water contents and volatile basic nitrogen of them were low. When defatting, the lightness and total phenol contents increased. When hot air dried, the crude fat contents were lower than that of freeze drying. It was suggested that in a large scale industries, the optimal condition combination(blanching, defatting, hot-air drying) to make a powder is more cost-effective and maintaining the quality of Protaetia brevitarsis larva powder. To produce Protaetia brevitarsis larva hydrolysate, the Protaetia brevitarsis larva powder manufactured by optimal processing was hydrolyzed using ultrasound treatment and enzymes (alcalase and neutrase). The appearance, yield, pH, degree of hydrolysis, solubility, SDS-PAGE, antioxidant activity (DPPH free radical scavenging activity, FRAP assay, ABTS radical scavenging, Hydrogen peroxide scavenging) of the hydrolysates were evaluated. Ultrasound treatment was effective to increase antioxidant activity and solubility of hydrolysate at two type enzymatic hydrolysis. In the alcalase hydrolysis, the initial hydrolysis rate also increased rapidly, and hydrolysate by alcalase were higher antioxidant activity than neutrase. During alcalase hydrolysis, it was observed that a low molecular weight peptide was produced according to the hydrolysis time. In this study, it was observed that bioactive peptides can be produced effectively by alcalase enzymatic hydrolysis and it was better utility when ultrasound assisted.