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흰점박이꽃무지 유충의 건조 및 단백가수분해물의 특성

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Author(s)
김하영
Publication Year
2021
URI
https://oak.jejunu.ac.kr/handle/2020.oak/23614
Abstract
This study was conducted to prepare a powder and protein hydrolysate from live Protaetia brevitarsis larva. In order to prepare the Protaetia brevitarsis larva powder, the manufacturing condition was variously designed with sacrifice method (blanching or freezing at –80℃), defatting process (defat, non-defat), storage temperature (-20℃, -80℃) and drying method (hot-air drying, freeze drying). And then we evaluated the appearance, yield, moisture contents, pH, color, proximate analysis, volatile basic nitrogen, DPPH free radical scavenging activity and total phenol contents of Protaetia brevitarsis larva powder. The optimal condition was the combination of blanching, defatting and -20℃ storage temperature and hot-air drying processes. When blanching the Protaetia brevitarsis larva, water contents and volatile basic nitrogen of them were low. When defatting, the lightness and total phenol contents increased. When hot air dried, the crude fat contents were lower than that of freeze drying. It was suggested that in a large scale industries, the optimal condition combination(blanching, defatting, hot-air drying) to make a powder is more cost-effective and maintaining the quality of Protaetia brevitarsis larva powder. To produce Protaetia brevitarsis larva hydrolysate, the Protaetia brevitarsis larva powder manufactured by optimal processing was hydrolyzed using ultrasound treatment and enzymes (alcalase and neutrase). The appearance, yield, pH, degree of hydrolysis, solubility, SDS-PAGE, antioxidant activity (DPPH free radical scavenging activity, FRAP assay, ABTS radical scavenging, Hydrogen peroxide scavenging) of the hydrolysates were evaluated. Ultrasound treatment was effective to increase antioxidant activity and solubility of hydrolysate at two type enzymatic hydrolysis. In the alcalase hydrolysis, the initial hydrolysis rate also increased rapidly, and hydrolysate by alcalase were higher antioxidant activity than neutrase. During alcalase hydrolysis, it was observed that a low molecular weight peptide was produced according to the hydrolysis time. In this study, it was observed that bioactive peptides can be produced effectively by alcalase enzymatic hydrolysis and it was better utility when ultrasound assisted.
This study was conducted to prepare a powder and protein hydrolysate from live Protaetia brevitarsis larva. In order to prepare the Protaetia brevitarsis larva powder, the manufacturing condition was variously designed with sacrifice method (blanching or freezing at –80℃), defatting process (defat, non-defat), storage temperature (-20℃, -80℃) and drying method (hot-air drying, freeze drying). And then we evaluated the appearance, yield, moisture contents, pH, color, proximate analysis, volatile basic nitrogen, DPPH free radical scavenging activity and total phenol contents of Protaetia brevitarsis larva powder. The optimal condition was the combination of blanching, defatting and -20℃ storage temperature and hot-air drying processes. When blanching the Protaetia brevitarsis larva, water contents and volatile basic nitrogen of them were low. When defatting, the lightness and total phenol contents increased. When hot air dried, the crude fat contents were lower than that of freeze drying. It was suggested that in a large scale industries, the optimal condition combination(blanching, defatting, hot-air drying) to make a powder is more cost-effective and maintaining the quality of Protaetia brevitarsis larva powder. To produce Protaetia brevitarsis larva hydrolysate, the Protaetia brevitarsis larva powder manufactured by optimal processing was hydrolyzed using ultrasound treatment and enzymes (alcalase and neutrase). The appearance, yield, pH, degree of hydrolysis, solubility, SDS-PAGE, antioxidant activity (DPPH free radical scavenging activity, FRAP assay, ABTS radical scavenging, Hydrogen peroxide scavenging) of the hydrolysates were evaluated. Ultrasound treatment was effective to increase antioxidant activity and solubility of hydrolysate at two type enzymatic hydrolysis. In the alcalase hydrolysis, the initial hydrolysis rate also increased rapidly, and hydrolysate by alcalase were higher antioxidant activity than neutrase. During alcalase hydrolysis, it was observed that a low molecular weight peptide was produced according to the hydrolysis time. In this study, it was observed that bioactive peptides can be produced effectively by alcalase enzymatic hydrolysis and it was better utility when ultrasound assisted.
Alternative Title
Characterization of Protaetia brevitarsis larva powder and enzymatic protein hydrolysates
Affiliation
제주대학교 대학원
Department
대학원 식품공학과
Advisor
천지연
Awarded
2021. 2
Table Of Contents
ABSTRACT 1
LIST OF FIGURES 2
LIST OF TABLES 3
1. 서론 5
2. 재료 및 방법 8
2.1. 흰점박이꽃무지 유충 분말 제조 8
2.2. 초음파 전처리 유무에 따른 효소 가수분해물 제조 10
2.3. 분석방법 12
2.3.1. 외관 및 색도 12
2.3.2. 수율 12
2.3.3. pH 12
2.3.4. 일반성분 12
2.3.5. 휘발성 염기 질소 화합물 12
2.3.6. 가수분해도 13
2.3.7. 용해도 13
2.3.8. 전기영동 14
2.3.9. 구성아미노산 14
2.3.10. 총 폴리페놀 함량 14
2.3.11. DPPH radical scavenging activity 14
2.3.12. ABTS radical scavenging activity 15
2.3.13. Hydrogen Peroxide scavenging activity 15
2.3.14. Ferric Ion Reducing Antioxidant Power (FRAP) 15
2.3.15. 통계처리 16
3. 결과 및 고찰 17
3.1. 전처리와 건조법에 따른 흰점박이꽃무지 유충 제조 17
3.1.1. 외관 17
3.1.2. 탈지수율 19
3.1.3. pH와 색도 21
3.1.4. 휘발성 염기 질소 화합물 25
3.1.5. 총 폴리페놀 함량 26
3.1.6. DPPH radical scavenging activity 27
3.1.7. 일반성분 30
3.2. 초음파 전처리 유무에 따른 흰점박이꽃무지 유충 분말 효소가 수분해물 제조 33
3.2.1. 외관 33
3.2.2. 수율 36
3.2.3. pH 38
3.2.4. 가수분해도 40
3.2.5. 용해도 42
3.2.6. DPPH radical scavenging activity 44
3.2.7. ABTS radical scavenging activity 46
3.2.8. Ferric Ion Reducing Antioxidant Power (FRAP) 48
3.2.9. Hydrogen Peroxide scavenging activity 50
3.2.10. 전기영동 52
3.2.11. 구성아미노산 54
4. 결론 56
국문요약 57
REFERENCES 59
Degree
Master
Publisher
제주대학교 대학원
Citation
김하영. (2021). 흰점박이꽃무지 유충의 건조 및 단백가수분해물의 특성
Type
Dissertation
Appears in Collections:
General Graduate School > Food science and Engineering
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